Yu, C.H., Luo J., Iwata-Reuyl D., Olsthoorn R.C. “Exploiting preQ(1) Riboswitches To Regulate Ribosomal Frameshifting.” ACS Chem Biol. 2013 Jan 25 PMID: 23327288

Chikwana, V. C., Stec, B., Lee, B. W. K., de Crécy-Lagard, V., Iwata-Reuyl, D., Swairjo, M. A. “Structural Basis of Biological Nitrile Reduction.” J. Biol. Chem. 2012, 287(36):30560-70 (PMID: 22787148, PMCID: PMC3436371)

Deutsch C., El Yacoubi B., de Crécy-Lagard V., Iwata-Reuyl D. “Biosynthesis of Threonylcarbamoyl Adenosine (t6A), a Universal tRNA Nucleoside.” J. Biol. Chem. 2012, 287(17):13666-73

Phillips, G., Swairjo, M.A., Gaston, K. W, Bailly, M., Limbach, P. A., Iwata-Reuyl, D., de Crécy-Lagard, V., “Diversity of Archaeosine Synthesis in Crenarchaeota.” ACS Chem. Biol. 2012, 7(2):300-5.

Phillips, G., Grochowski, L. L., Bonnett, S., Xu, H., Bailly, M., Blaby-Haas, C., El Yacoubi, B., Iwata-Reuyl, D., White, R. H., de Crécy-Lagard, V. 
”Functional Promiscuity of the COG0720 Family.” ACS Chem. Biol. 2012, 7(1):197-209.

Rakovich T., Boland C., Bernstein I., Chikwana V. M., Iwata-Reuyl D., Kelly V. P. “Queuosine deficiency in eukaryotes compromises tyrosine production through increased tetrahydrobiopterin oxidation.” J. Biol. Chem. 2011, 286(22):19354-19363.

El Yacoubi, B., Hatin, I., Deutsch, C., Kahveci, T., Rousset, J-P., Iwata-Reuyl, D., Murzin, A., and de Crécy-Lagard, V. “A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification.” EMBO J. 2011, 30(5):882-893.

Phillips, G., Chikwana, V. M., Maxwell, A., El-Yacoubi, B., Swairjo, M. A., Iwata-Reuyl, D. and de Crécy-Lagard, V. “Discovery and characterization of an amidinotransferase involved in the modification of archaeal tRNA.” J. Biol. Chem. 2010, 285:17, 12706-12713.

Sankaran, B.; Bonnett, S.; Shah, K.; Gabriel, S.; Reddy, R.; Schimmel, P.; Rodionov, D. A.; de Crécy-Lagard, V.; Helmann, J. D.; Iwata-Reuyl, D.; Swairjo, M. A. "Zinc-independent folate biosynthesis: genetic, biochemical, and structural investigations reveal new metal dependence for GTP Cyclohydrolase IB," J. Bacteriol., 2009, 191, 6939-6949.

Iwata-Reuyl, D., and de Crécy-Lagard, V. 2009. "Enzymatic Formation of the 7-Deazaguanosine Hypermodified Nucleosides of tRNA," pp. 377-391. InDNA and RNA Modification Enzymes: Structure, Mechanism, Function and Evolution, Henri Grosjean, Ed. Landes Bioscience, Austin, Tx.

Phillips, G.; El Yacoubi, B.; Lyons, B.; Alvarez, S.; Iwata-Reuyl, D.; de Crécy-Lagard, V. "The biosynthesis of the 7-deazaguanosine modified tRNA nucleosides: a new role for GTP cyclohydrolase I," J. Bacteriol., 2008, 190, 7876-7884.

Iwata-Reuyl, D. "An Embarassment of Riches: The enzymology of tRNA modification," Current Opinion in Chemical Biology, 2008,12, 126-133.

Lee, B.; Van Lanen, S. G.; Iwata-Reuyl, D. "Mechanistic studies of QueF, the nitrile oxidoreductase involved in the biosynthesis of queuosine," Biochemistry,2008, 46, 12844-12854.

Roth, A.; Winkler, W. C.; Regulski, E.; Lee, B.; Lim, J.; Jona, I.; Barrick, J. E.; Ritwik, A.; Kim, J.; Welz, R.; Iwata-Reuyl, D.; Breaker, R. R. "A eubacterial riboswitch selective for the queuosine precursor preQ1 contains an unusually small aptamer domain," Nature Struct. Mol. Biol., 2007, 14, 308-317.

El Yacoubi, B.; Bonnett, S.; Anderson, J.; Swairjo, M. A.; Iwata-Reuyl, D. and de Crécy-Lagard, V. "Discovery of a new prokaryotic type I GTP cyclohydrolase family," J. Biol. Chem., 2006, 281(49), 37586-37593.

Swairjo, M. A.; Reddy, R. R.; Lee, B.; Van Lanen, S. G.; Brown, S.; de Crécy-Lagard, V.; Iwata-Reuyl, D.; and Schimmel, P. "Crystallization and preliminary X-ray characterization of the nitrile reductase QueF–a queuosine biosynthesis enzyme," Acta Cryst., 2005, F61, 945-948.

Overbeek, R., et al. "The Subsystems Approach to Genome Annotation and its Use in the Project to Annotate 1,000 Genomes," Nucleic Acids Res., 2005, 33(17), 5691-5702.

Van Lanen, S. G.; Reader, J. S.: Swairjo, M. A..; de Crécy-Lagard, V.; Lee, B.; Iwata-Reuyl, D., "From cyclohydrolase to oxido-reductase: Discovery of nitrile reductase activity in a common fold," Proc. Natl. Acad. Sci. USA, 2005, 102:12, 4264-4269.

Van Lanen, S.; Iwata-Reuyl, D., "Kinetic Mechanism of the tRNA Modifying Enzyme S-Adenosylmethionine:tRNA Ribosyltransferase-isomerase (QueA)," Biochemistry, 2003, 42:18, 5312-5320.

Van Lanen, S.; Daoud Kinzie, S.; Matthieu, S.; Link, T.; Culp, J.; Iwata-Reuyl, D., "tRNA Modification by S-Adenosylmethionine:tRNA Ribosyltransferase-isomerase (QueA): Assay Development and Characterization of the Recombinant Enzyme," J. Biol. Chem., 2003, 278:12, 10491-10499.

Iwata-Reuyl, D., "The Biosynthesis of the 7-Deazaguanosine Hypermodified Nucleosides of Transfer RNA," Bioorg. Chem., 2003, 31, 24-43.

Bai, Y.; Fox, D.; Lacy, J.; Van Lanen, S.; and Iwata-Reuyl, D. "Hypermodification of tRNA in Thermophilic Archae: Cloning, Over-Expression, and Characterization of tRNA-Guanine Transglycosylase from Methanococcus jannaschii," J. Biol. Chem., 2000, 275:37, 28731-28738.

Daoud Kinzie, S.; Thern, B.; Iwata-Reuyl, D. "Mechanistic Studies of the tRNA-Hypermodifying Enzyme QueA: A Chemical Imperative for the Use of AdoMet as a 'Ribosyl' Donor," Org. Lett. 2000, 2, 1307-1310.



February 2010  Congratulations to Vimbai Chikwana, who's paper on the discovery of ArcS was accepted for publication in JBC and selected to be highlighted as a "Paper of the Week." Publication date April 23rd.

June 2009: Congratulations are in order for Vimbai Chikwani, who was awarded an inaugural William Paudler graduate fellowship, Kelly Chaçon, who was named the outstanding graduating senior from the Department of Chemistry, and Arjay Ercolani, who was name Analytical Chemistry Student of the year from the Department of Chemistry.

April 2009: Welcome to Ben Turner, who is joining the lab as a senior scientist. Ben received his B.S. in Chemistry from the University of Dundee in Scotland, and worked for several years on the characterization of the RNA K-turn with David Lilley before crossing the pond to the states.

March 2009: Congratulations to Xander Taylor for his successful grant application to the MJ Murdock Charitable Trust. Xander is a high school teacher at West Linn High School, and will be supported for 2 summers in the lab through the Partners in Science program beginning summer '09.

March 2009: Congratulations to Kelly Chaçon for her award of a NASA Oregon Space Grant Scholarship.

Past News...

    2009-10 Iwata-Reuyl Lab