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Organic
Chemistry III
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Professor
Carl C. Wamser
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Chapter
27 Skills
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Amino Acids and Proteins
Knowledge - Level 1
know the terminology:
amino acid, essential amino acid, amino acid residue
peptide, polypeptide, protein, enzyme
N-terminus, C-terminus, alpha-helix, beta-sheet
zwitterion, isoelectric point, electrophoresis
know the structures of the 20 common amino acids by name and 3-letter
abbreviation
know the approximate pKa values for the amino acids
Concepts - Level 2
understand the relationship between the pKa values of amino acids and
peptides and the different ionization states they may assume in solutions
depending on the pH
understand the classification systems for proteins
based on composition (simple or conjugated)
based on shape and solubility (fibrous (insoluble) or globular (soluble))
based on function (enzymes, structural, etc.)
understand the classification system for protein structural features:
primary: amino acid sequence
secondary: chain conformations (alpha-helix, beta-sheet)
tertiary: 3-d structure of the entire protein
quaternary: interactions of multiple polypeptide chains
recognize the various reactions of amino acids and peptides:
peptide bonding
disulfide bonding
ninhydrin test
Edman degradation
BOC protecting group
cleavage by chymotrypsin and trypsin
Applications - Level 3
identify the expected state of ionization of amino acids or peptides
based on a given pH and their pKa values
name and give abbreviations for simple peptides
indicate the steps necessary for peptide sequencing
indicate the steps necessary for a peptide synthesis
Analytical Skills - Level 4
identify features that contribute to the various levels of protein structure
determine the amino acid sequence of a polypeptide based on partial hydrolysis
and other results
