Hardware for NAI-ViFoG MonoMammoth fieldtrip –

Ray will bring:

1. Apparatus for large-volume sample filtration

• a. Submersible pump (runs off 12 V DC w/ max flow 13.8 L/min)
• b. Tubing (sufficient to obtain samples from depth down to 5 m), fittings & wound polypropylene filter cartridges (100, 10, & 1 mm)
• c. Opticap XL4 low-protein binding filter capsules (0.5/0.2 mm nominal)
• d. Container & container-liners for collection of 100 L samples

2. Apparatus for tangential flow filtration (TFF)

• a. Pellicon 2 TFF system for concentrating >30 kD fraction
  • i. Peristaltic pump (115 V AC)
    • 1. will need portable generator or alternative for use in field
  • ii. Flow rate w/ system will be approx. 0.5L/min
    • 1. Allows concentration of 100 L of prefiltered lake or spring water down to 100 mL retentate in approx. 3.5 h.

3. HydroLab Datasonde 4a multiprobe w/ 50 m of cable

• a. For measurement of depth, pH, temp. DO, specific conductance, oxygen reduction potential, photosynthetically-active radiation, turbidity

Various sample bottles & tubes, sterile disposable syringes and syringe filters for filter-sterilization of viral concentrates, freezer-paks, chemical heat-paks and insulated coolers for sample transport

Most desired item:

• 1. a portable power source (small gas-powered generator?) that can be used to run the peristaltic pump(s) in the field

Forest will bring:

• A generator, peristaltic pump, and filters.

Ken will bring:

• GPS, topo maps, hand vacuum pump, long extendible (12ft) pole for collecting samples, Infra-red thermometer, conventional thermocouple thermometer.

David will bring:

• a battery, peristaltic pump, and filter.

Mark will bring:

• A flourescent microscope.
• Standard sampling gear
• A laptop projector