| Strain: CL | Date of Entry | Other Names | Parental Strain | Source Person | Agent | Change from Parental | Genotype | Plasmid | F | Transposon | Reference | Location | species |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 001 | 7/6/2001 | HL812, SR108 | W3110 | Justin Courcelle | trimethoprim low thy | thy- | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph (pyrimidine starvation)? see J Bact 175, 3401-3407 (1993) Should be the same as HL108 and HL812, but received from J C Courcelle as the direct parent of his strains HL919-925, and 928. Nature 224, 1164-1166 (1969) original |
no | no | no | Box1, A1 | E. coli K-12 | |
| 002 | 7/6/2001 | HL921 | SR108 (=HL930) | Justin Courcelle | Transduction P1 from JC10289 (=HL456) | recA::tetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, D(srlR- recA)306::Tn10, rph? see J Bact 175, 3401-3407 (1993) Transduction with P1 from JC10289 (=HL456) Mol Gen Genet 183, 497-504 (1981); |
no | no | Tn10 | Box1, A2 | E. coli K-12 | |
| 003 | 7/6/2001 | HL922 | SR108 (=HL930) | Justin Courcelle | Transduction with P1 from V1307 (=HL932) | TetR (recBC) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recB21, recC22, argA81::Tn10, rph? see J Bact 175, 3401-3407 (1993) Transduction with P1 from V1307 (=HL932) Proc Nat Acad Sci 81, 7850-7854 (1984) |
no | no | Tn10 | Box1, A3 | E. coli K-12 | |
| 004 | 7/6/2001 | HL923 | SR108 (=HL930) | Justin Courcelle | Transduction with P1 from V220 (=HL931) | TetR (recD) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recD1011, argA81::Tn10, rph? see J Bact 175, 3401-3407 (1993) NB: recD1011 was originally designated recB1011 Transduction with P1 from V220 (=HL931) Proc Nat Acad Sci 81, 7850-7854 (1984) |
no | no | Tn10 | Box1, A4 | E. coli K-12 | |
| 005 | 7/6/2001 | HL919 | SR108 (=HL930) | Justin Courcelle | Transduction with P1 from JC15359 (HL816) | TetR (recF, tnaA) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recF349, tnaA300::Tn10, rph? see J Bact 175, 3401-3407 (1993) Proc Nat Acad Sci 81, 7850-7854 (1984) |
no | no | Tn10 | Box1, A5 | E. coli K-12 | |
| 006 | 7/6/2001 | HL920 | SR108 (=HL930) | Justin Courcelle | Transduction with P1 from JC15359 (HL816) | KanR (recR) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recR252::Tn10-9, rph? see J Bact 175, 3401-3407 (1993) J Bact 176, 3661-3672 (1994); Proc Nat Acad Sci 81, 7850-7854 (1984) |
no | no | Tn10-9 | Box1, A6 | E. coli K-12 | |
| 007 | 7/6/2001 | HL946 | SR108 (=HL930) | Justin Courcelle | Transduction with P1 from HL556 | Tn3 (AmpR) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3 J Bact 181, 916-922 (1999); also found tis to be TetR. So it carried in tnaA300::Tn10 frm HL556 during the transduction as well |
no | no | Tn3 (AmpR) Tn10 tetR | Box1, A7 | E. coli K-12 | |
| 008 | 7/6/2001 | HL945 | SR108 (=HL930) | Justin Courcelle | Transduction with P1 from HL962 | recG (KanR) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recG::Tn5 | no | no | Tn5 (KanR) | Box1, A8 | E. coli K-12 | |
| 009 | 7/6/2001 | HL944 | SR108 (=HL930) | Justin Courcelle | Transduction with P1 from HL956 | recQ (ampR) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recQ1803::Tn3 | no | no | Tn3 (AmpR) | Box1, A9 | E. coli K-12 | |
| 010 | 7/6/2001 | HL924 | SR108 (=HL930) | Justin Courcelle | Transduction with P1 from JC12123 | TetR (recJ) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recJ284::Tn10, rph? J Bact 157, 190-196 (1984) |
no | no | Tn10 (TetR) | Box1, B1 | E. coli K-12 | |
| 011 | 7/6/2001 | HL950 | HL919 | Justin Courcelle | Transduction with P1 from HL962 | recG::Tn5 | thyA36, deoC2, IN(rrnD-rrnE)1, recF349, tnaA300::Tn10, rph?, recG::Tn5 |
no | no | Tn10 TetR, Tn5 KanR | Box1, B2 | E. coli K-12 | |
| 012 | 7/6/2001 | HL956 | HL919 | Justin Courcelle | Transduction with P1 from HL962 | recQ::Tn3 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recF349, tnaA300::Tn10, rph?, recQ1803::Tn3 | no | no | Tn10 TetR, Tn3 ampR | Box1, B3 | E. coli K-12 | |
| 013 | 7/6/2001 | HL948 | HL919 | Justin Courcelle | Transduction with P1 from HL956 | kanR mfd | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recF349, tnaA300::Tn10, rph?, mfd::Tn5 | no | no | Tn10TetR, Tn5KanR | Box1, B4 | E. coli K-12 | |
| 014 | 7/6/2001 | HL947 | HL946 | Justin Courcelle | Transduction with P1 from HL962 | recG::Tn5 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3, recG::Tn5 |
no | no | Tn3 ampR, Tn5 KanR | Box1, B5 | E. coli K-12 | |
| 015 | 7/6/2001 | HL968 | HL920 | Justin Courcelle | Transduction with P1 from HL931(V220) | recD::Tn10 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recR252::Tn10-9, recD1011, argA81::Tn10, rph? see J Bact 175, 3401-3407 (1993) NB: recD1011 was originally designated recB1011 Proc Nat Acad Sci 81, 7850-7854 (1984); |
no | no | Tn10 tetR, Tn10-9 KanR | Box1, B6 | E. coli K-12 | |
| 016 | 7/6/2001 | HL969 | HL920 | Justin Courcelle | Transduction with P1 from HL960 | recJ284::Tn10 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recR252::Tn10-9, recJ284::Tn10 rph? see J Bact 175, 3401-3407 (1993) |
no | no | Tn10 tetR, Tn10-9 KanR | Box1, B7 | E. coli K-12 | |
| 017 | 7/6/2001 | HL920 | Justin Courcelle | Transduction with P1 from HL456 | D(srlR- recA)306::Tn10 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recR252::Tn10-9, D(srlR- recA)306::Tn10 rph? see J Bact 175, 3401-3407 (1993) |
no | no | Tn10 tetR, Tn10-9 KanR | Box1, B8 | E. coli K-12 | ||
| 018 | 7/6/2001 | HL928 | HL930 | Justin Courcelle | transduction with P1 from BW560 (=HL670) | DTetR (ung-) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, ung-152::Tn10, rph? see J Bact 175, 3401-3407 (1993) | no | no | Tn10 tetR | Box1, B9 | E. coli K-12 | |
| 019 | 7/6/2001 | HL939 | HL930 | dj crowley | transduction with P1 from HL667 | mfd::kan | l-, thyA36, deoC2, IN(rrnD-rrnE)1, mfd::kan | no | no | kanR | Box1, C1 | E. coli K-12 | |
| 020 | 7/6/2001 | HL959 | HL944 | JC Courcelle | transduction with P1 from HL818 | mfd::kan | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recQ1803::Tn3, recR252::Tn10-9 | no | no | Tn3 (AmpR), Tn5 (KanR) | Box1, C2 | E. coli K-12 | |
| 021 | 3/14/2002 | HL952 | HL930 | Justin Courcelle | Transduction with P1 from HL758 | TetR | l-, uvrA::Tn10, thyA36, deoC2, IN(rrnD-rrnE)1, rph? see J Bact 175, 3401-3407 (1993) |
no | no | Tn10 (TetR) | Box1, C3 | E. coli K-12 | |
| 022 | 3/15/2002 | HL925 | HL930 | Justin Courcelle | Transduction with P1 from HL765 | TetR uvrC | l-, uvrC297::Tn10, thyA36, deoC2, IN(rrnD-rrnE)1, rph? see J Bact 175, 3401-3407 (1993) |
no | no | Tn10 (TetR) | Box1, C4 | E. coli K-12 | |
| 023 | 3/15/2002 | HL972 | HL930 | Justin Courcelle | Transduction with P1 from HL759 | KanR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, uvrA6, zjd::Tn5, |
no | no | Tn5 (kanR) | Box1, C5 | E. coli K-12 | |
| 024 | 3/15/2002 | HL955 | HL944 | Justin Courcelle | Transduction with P1 from HL759 | KanR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recQ1803::Tn3, uvrA6, zjd::Tn5, |
no | no | Tn5 (kanR) Tn3 (ampR) | Box1, C6 | E. coli K-12 | |
| 025 | 3/15/2002 | HL958 | HL946 | Justin Courcelle | Transduction with P1 from HL759 | KanR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF322::Tn3, uvrA6, zjd::Tn5, |
no | no | Tn5 (kanR) Tn3 (ampR) | Box1, C7 | E. coli K-12 | |
| 026 | 3/15/2002 | HL966 | HL920 | Justin Courcelle | Transduction with P1 from HL758 | KanR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recR252::Tn10-9, uvrA::Tn10 rph? see J Bact 175, 3401-3407 (1993) |
no | no | Tn10-9 (kanR) Tn10 (tetR) | Box1, C8 | E. coli K-12 | |
| 027 | 3/15/2002 | HL965 | HL919 | Justin Courcelle | Transduction with P1 from HL759 | KanR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recF349, tnaA300::Tn10, uvrA6, zjd::Tn5, rph? see J Bact 175, 3401-3407 (1993) |
no | no | Tn5 (kanR) Tn10 (tetR) | Box1, C9 | E. coli K-12 | |
| 028 | 3/15/2002 | HL964 | HL924 | Justin Courcelle | Transduction with P1 from HL759 | KanR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recJ284::Tn10, uvrA6, zjd::Tn5, rph? see J Bact 175, 3401-3407 (1993) |
no | no | Tn5 (kanR) Tn10 (tetR) | Box1, D1 | E. coli K-12 | |
| 029 | 3/15/2002 | HL967 | HL920 | Justin Courcelle | Transduction with P1 from HL765 | TetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recR252::Tn10-9, uvrC279::Tn10 rph? see J Bact 175, 3401-3407 (1993) |
no | no | Tn10-9 (kanR) Tn10 (tetR) | Box1, D2 | E. coli K-12 | |
| 030 | 3/15/2002 | HL973 | HL946 | Justin Courcelle | Transduction with P1 from HL960 | TetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3, recJ284::Tn10 |
no | no | Tn3 (ampR) Tn10 (tetR) | Box1, D3 | E. coli K-12 | |
| 031 | 3/15/2002 | HL974 | HL944 | Justin Courcelle | Transduction with P1 from HL765 | TetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recQ1803::Tn3, uvrC279::Tn10 |
no | no | Tn3 (ampR) Tn10 (tetR) | Box1, D4 | E. coli K-12 | |
| 032 | 3/15/2002 | HL975 | HL946 | Justin Courcelle | Transduction with P1 from HL758 | TetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3, uvrA::Tn10 |
no | no | Tn3 (ampR) Tn10 (tetR) | Box1, D5 | E. coli K-12 | |
| 033 | 3/15/2002 | HL929 | HL920 | Justin Courcelle | Transduction with P1 from HL760 | TetR ung- | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recR252::Tn10-9, ung-152::Tn10, rph? see J Bact 175, 3401-3407 (1993) |
no | no | Tn10-9 (kanR) Tn10 (tetR) | Box1, D6 | E. coli K-12 | |
| 034 | 3/15/2002 | HL976 | HL946 | Justin Courcelle | Transduction with P1 from HL765 | TetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3, uvrC279::Tn10 |
no | no | Tn3 (ampR) Tn10 (tetR) | Box1, D7 | E. coli K-12 | |
| 035 | 3/15/2002 | HL1000 | HL930 | Justin Courcelle | Transduction with P1 from HL971 | kanR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, Dtus::Tn5 rph? see J Bact 175, 3401-3407 (1993) |
no | no | Tn5 (kanR) | Box1, D8 | E. coli K-12 | |
| 036 | 3/15/2002 | HL1001 | HL1000 | Justin Courcelle | Transduction with P1 from HL932 | tetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, Dtus::Tn5, recB21, recC22, argD81::Tn10 rph? see J Bact 175, 3401-3407 (1993) |
no | no | Tn5 (kanR) Tn10 (tetR) | Box1, D9 | E. coli K-12 | |
| 037 | 3/15/2002 | HL1002 | HL1000 | Justin Courcelle | Transduction with P1 from HL931 | tetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, Dtus::Tn5, recD1011, argD81::Tn10 rph? see J Bact 175, 3401-3407 (1993) |
no | no | Tn5 (kanR) Tn10 (tetR) | Box1, E1 | E. coli K-12 | |
| 038 | 3/15/2002 | HL1002 | HL1000 | Justin Courcelle | Transduction with P1 from HL931 | tetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, Dtus::Tn5, recD1011, argD81::Tn10 rph? see J Bact 175, 3401-3407 (1993) a different isolate from the same transduction as CL037 ***this isolate however grows “sick” whereas CL037 seems to be healthy. Not sure which is correct. perhaps a supressor in CL037??? |
no | no | Tn5 (kanR) Tn10 (tetR) | Box1, E2 | E. coli K-12 | |
| 039 | 3/15/2002 | HL1034 | HL930 | Justin Courcelle | Transduction with P1 from HL1033 | DxonA::cat300 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, DxonA::cat300 rph? see J Bact 175, 3401-3407 (1993) |
no | no | Box1, E3 | E. coli K-12 | ||
| 040 | 3/15/2002 | HL1035 | HL946 | Justin Courcelle | Transduction with P1 from HL1033 | DxonA::cat300 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3, DxonA::cat300 |
no | no | Tn3 ampR | Box1, E4 | E. coli K-12 | |
| 041 | 3/15/2002 | HL1048 | HL1046 | Justin Courcelle | Transduction with P1 from HL556 | AmpR (RecF) | l-, IN(rrnD-rrnE)1, D(thyA::kanR), recF332::Tn3 rph? see J Bact 175, 3401-3407 (1993) |
no | no | Tn3 ampR | Box1, E5 | E. coli K-12 | |
| 042 | 3/15/2002 | HL1047 | HL1046 | Justin Courcelle | Transduction with P1 from HL683 | Tn10 tetR | l-, IN(rrnD-rrnE)1, D(thyA::kanR), ung152::Tn10 rph? see J Bact 175, 3401-3407 (1993) |
no | no | Tn10 tetR | Box1, E6 | E. coli K-12 | |
| 043 | 3/15/2002 | MG1655 | Courcelle via A.Khodurski | "wild type" (but l-) "It contains a frameshift mutation at the end of rph, causing low expression of the downstream gene pyrE and, in turn, a pyrimidine starvation phenotype. " used for genomic microarrays Science 277, 1453-1474 (1997); |
no | no | no | Box1, E7 | E. coli K-12 | ||||
| 044 | 3/15/2002 | MG1655 | Courcelle via A.Khodurski | "wild type" (but l-) "It contains a frameshift mutation at the end of rph, causing low expression of the downstream gene pyrE and, in turn, a pyrimidine starvation phenotype. " used for genomic microarrays Science 277, 1453-1474 (1997); same as CL044 different isolate from same plate |
no | no | no | Box1, E8 | E. coli K-12 | ||||
| 045 | 3/15/2002 | CL004 | J Courcelle | P1 transduction from HL1033 | DxonA::cat300 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recD1011, argA81::Tn10, rph? DxonA::cat300 see J Bact 175, 3401-3407 (1993) |
no | no | Tn10 | Box1, E9 | E. coli K-12 | ||
| 046 | 3/15/2002 | M15[pREP4] | J Courcelle | ac, ara, gal, mtl, (recA+) M15 is the name of a lacZ deletion (J Mol Biol 8, 427-430 (1964); not sure whether the lac- mutation in this strain is D(lacZ)15. Genotype above is given in QIAexpressionist (1992). pREP4 (carries lacI; results in high levels of lac repressor protein; compatible with plasmids with ColE1 origin; use 25 µg/ml kanamycin for selection) pMALR4 is pMALp2 with the recR gene cloned into the polylinker. It complements a recR mutant for UV snesitivity. |
pMALR4 | no | no | Box1, F1 | E. coli K-12 | ||||
| 047 | 7/6/2001 | HL921 | SR108 (=HL930) | Justin Courcelle | Transduction P1 from JC10289 (=HL456) | recA::tetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, D(srlR- recA)306::Tn10, rph? see J Bact 175, 3401-3407 (1993) Transduction with P1 from JC10289 (=HL456) Mol Gen Genet 183, 497-504 (1981); should be the same as CL002 |
no | no | Tn10 | Box1, F2 | E. coli K-12 | |
| 048 | 3/15/2002 | HL1047 | HL1046 | Justin Courcelle | Transduction with P1 from HL683 | Tn10 tetR | l-, IN(rrnD-rrnE)1, D(thyA::kanR), ung152::Tn10 rph? see J Bact 175, 3401-3407 (1993) should be the same as CL042 but different isolate |
no | no | Tn10 tetR | Box1, F3 | E. coli K-12 | |
| 049 | 3/15/2002 | HL1048 | HL1046 | Justin Courcelle | Transduction with P1 from HL556 | AmpR (RecF) | l-, IN(rrnD-rrnE)1, D(thyA::kanR), recF332::Tn3 rph? see J Bact 175, 3401-3407 (1993) should be the same as CL041 but different isolate |
no | no | Tn3 ampR | Box1, F4 | E. coli K-12 | |
| 050 | 3/15/2002 | HL1046 | W3110 | Justin Courcelle | Transduction with P1 from HL771 | kan thyA | l-, IN(rrnD-rrnE)1, D(thyA::kanR) rph? see J Bact 175, 3401-3407 (1993) |
no | no | Box1, F5 | E. coli K-12 | ||
| 051 | 3/15/2002 | HL1046 | W3110 | Justin Courcelle | Transduction with P1 from HL771 | kan thyA | l-, IN(rrnD-rrnE)1, D(thyA::kanR) rph? see J Bact 175, 3401-3407 (1993) should be the same as CL051 but different isolate |
no | no | Box1, F6 | E. coli K-12 | ||
| 052 | 3/15/2002 | M15[pREP4] | J Courcelle | transformation | ac, ara, gal, mtl, (recA+) M15 is the name of a lacZ deletion (J Mol Biol 8, 427-430 (1964); not sure whether the lac- mutation in this strain is D(lacZ)15. Genotype above is given in QIAexpressionist (1992). pREP4 (carries lacI; results in high levels of lac repressor protein; compatible with plasmids with ColE1 origin; use 25 µg/ml kanamycin for selection) pMALp2 is an expression vector that has a polylinker cloning site with a Maltose Binding Protein tag in it. |
pMALp2, pREP4 | no | no | Box1, F7 | E. coli K-12 | |||
| 053 | 3/15/2002 | HL1002 | HL1000 | J Courcelle | Transduction with P1 from HL931 | tetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, Dtus::Tn5, recD1011, argD81::Tn10 rph? see J Bact 175, 3401-3407 (1993) |
no | Tn10 (TetR) | Box1, F8 | E. coli K-12 | ||
| 054 | 3/15/2002 | CL004 | J Courcelle | P1 transduction from HL956 | Tn3 ampR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recQ1803::Tn3 recD1011, argA81::Tn10 |
no | Tn3 ampR Tn10 tetR | Box1, F9 | E. coli K-12 | |||
| 055 | 3/15/2002 | HL205 CRT266 PCH5 | Y. Hirota | thr, leu, met, thyA, (deo?), tonA, dnaB266 ***temp sens*** confirmed temp sens, thy- and requirement for amino acids (casamino acids) 8/94 Ann Inst Pasteur 110, 465 |
no | no | Box1, G1 | E. coli K-12 | |||||
| 056 | 3/15/2002 | HL205 | J Courcelle | p1 transduction from HL456 |
tetR Tn10 | thr, leu, met, thyA, (deo?), tonA, dnaB266, D(srlR- recA)306::Tn10 ***temp sens*** |
no | Tn10 | Box1, G2 | E. coli K-12 | |||
| 057 | 3/15/2002 | HL953 | HL205 | J Courcelle | p1 transduction from HL818 |
KanR recR252:: Tn10-9 | thr, leu, met, thyA, (deo?), tonA, dnaB266, recR252::Tn10-9 ***temp sens*** |
no | Tn10-9 | Box1, G3 | E. coli K-12 | ||
| 058 | 3/15/2002 | HL205 | J Courcelle | p1 transduction from HL817 |
KanR recO1504::tn5 | thr, leu, met, thyA, (deo?), tonA, dnaB266, recO1504::Tn5 ***temp sens*** |
no | Tn5 kanR | Box1, G4 | E. coli K-12 | |||
| 059 | 3/15/2002 | HL954 | HL205 | J Courcelle | p1 transduction from HL816 |
TetR | thr, leu, met, thyA, (deo?), tonA, dnaB266, recF349, tna300::Tn10 ***temp sens*** |
no | Tn10 tetR | Box1, G5 | E. coli K-12 | ||
| 060 | 3/15/2002 | CL057 | J Courcelle | p1 transduction from HL456 |
Tn10 tetR | thr, leu, met, thyA, (deo?), tonA, dnaB266, recR252::Tn10-9 D(srlR- recA)306::Tn10 ***temp sens*** |
no | Tn10-9 Tn10 | Box1, G6 | E. coli K-12 | |||
| 061 | 3/15/2002 | M15[pREP4] | J Courcelle | transformation amp selection | ac, ara, gal, mtl, (recA+) M15 is the name of a lacZ deletion (J Mol Biol 8, 427-430 (1964); not sure whether the lac- mutation in this strain is D(lacZ)15. Genotype above is given in QIAexpressionist (1992). pREP4 (carries lacI; results in high levels of lac repressor protein; compatible with plasmids with ColE1 origin; use 25 µg/ml kanamycin for selection) pMALF6 is pMALp2 with the recF gene cloned into the polylinker. It complements a recF mutant for UV snesitivity. |
pMALF6 | no | no | Box1, G7 | E. coli K-12 | |||
| 062 | 3/15/2002 | M15[pREP4] | J Courcelle | transformation amp selection | ac, ara, gal, mtl, (recA+) M15 is the name of a lacZ deletion (J Mol Biol 8, 427-430 (1964); not sure whether the lac- mutation in this strain is D(lacZ)15. Genotype above is given in QIAexpressionist (1992). pREP4 (carries lacI; results in high levels of lac repressor protein; compatible with plasmids with ColE1 origin; use 25 µg/ml kanamycin for selection) pMALF6 is pMALp2 with the recF gene cloned into the polylinker. It complements a recF mutant for UV snesitivity. should be the same as CL061 but different isolate |
pMALF6 | no | no | Box1, G8 | E. coli K-12 | |||
| 063 | 3/15/2002 | HL928 | J Courcelle | transduction with P1 from HL1058 | dut-21::cat | l-, thyA36, deoC2, IN(rrnD-rrnE)1,ung-152::Tn10, dut-21::cat, rph? see J Bact 175, 3401-3407 (1993) Not a great grower???? I worry about this strain. May want to reconstruct the mutations. Suppressors???? same as CL075 |
no | Box1, G9 | E. coli K-12 | ||||
| 064 | 3/15/2002 | AG11 E486 HL311 | J J. Wechsler | thr, leu, thi, thyA, deo, lac, rpsL, tonA, SuII+, dnaE486, (met?) **thermosensitive** Proc Nat Acad Sci 68, 3150-3153 (1971); Mol Gen Genet 113, 273-284 (1971) |
no | no | Box1, H1 | E. coli K-12 | |||||
| 065 | 3/15/2002 | HL1003 | HL311 | J Courcelle | P1 transduction from HL944 | ampR Tn3 | thr, leu, thi, thyA, deo, lac, rpsL, tonA, SuII+, dnaE486, (met?), recQ1803::Tn3 **thermosensitive** |
no | Tn3 ampR | Box1, H2 | E. coli K-12 | ||
| 066 | 3/15/2002 | HL990 | HL311 | J Courcelle | P1 transduction from HL960 | tetR recJ284 | thr, leu, thi, thyA, deo, lac, rpsL, tonA, SuII+, dnaE486, (met?), recJ284::Tn10 **thermosensitive** |
no | Tn10 tetR | Box1, H3 | E. coli K-12 | ||
| 067 | 3/15/2002 | HL989 | HL311 | J Courcelle | P1 transduction from HL818 | kanR recR252 | thr, leu, thi, thyA, deo, lac, rpsL, tonA, SuII+, dnaE486, (met?), recR252::Tn10-9 **thermosensitive** |
no | Tn10-9 kanR | Box1, H4 | E. coli K-12 | ||
| 068 | 3/15/2002 | HL988 | HL311 | J Courcelle | P1 transduction from HL556 | ampR recF332 | thr, leu, thi, thyA, deo, lac, rpsL, tonA, SuII+, dnaE486, (met?), recF332::Tn 3 **thermosensitive** |
no | Tn3 ampR | Box1, H5 | E. coli K-12 | ||
| 069 | 3/15/2002 | HL987 | HL311 | J Courcelle | P1 transduction from HL556 | tetR recF349 | thr, leu, thi, thyA, deo, lac, rpsL, tonA, SuII+, dnaE486, (met?), recF349, tna300::Tn10 **thermosensitive** |
no | Tn10 tetR | Box1, H6 | E. coli K-12 | ||
| 070 | 3/15/2002 | DH5alpha | J Courcelle via Ann Britt | transformation amp selection | pAT6-4phr | Ø80dlacZDM15, D(lacZYA-argF)U169, recA1, endA1, hsdR17 (rk- mk+), supE44, l-, thi-1, gyrA, relA1, phoA pAT6-4phr is a plasmid that contains a 6-4 photolyase from a plant which I cant rememer right now. It should be easily found in some of Ann Britts older papers. She sent us the plasmid |
no | Box1, H7 | E. coli K-12 | ||||
| 071 | 3/15/2002 | M15[pREP4] | J Courcelle | transformation amp selection | ac, ara, gal, mtl, (recA+) M15 is the name of a lacZ deletion (J Mol Biol 8, 427-430 (1964); not sure whether the lac- mutation in this strain is D(lacZ)15. Genotype above is given in QIAexpressionist (1992). pREP4 (carries lacI; results in high levels of lac repressor protein; compatible with plasmids with ColE1 origin; use 25 µg/ml kanamycin for selection) pMALO11 is pMALp2 with the recO gene cloned into the polylinker. It complements a recO mutant for UV snesitivity. |
pMALO11 | no | no | Box1, H8 | E. coli K-12 | |||
| 072 | 3/15/2002 | CL007 | J Courcelle | transformation from HL1058 | dut-21::cat | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3, dut-21::cat J Bact 181, 916-922 (1999); also found tis to be TetR. So it carried in tnaA300::Tn10 frm HL556 during the transduction as well |
no | no | Box1, H9 | E. coli K-12 | |||
| 073 | 3/15/2002 | HL928 | HL930 | J Courcelle | transduction with P1 from BW560 (=HL670) | TetR (ung-) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, ung-152::Tn10, rph? see J Bact 175, 3401-3407 (1993) Not a great grower???? I worry about this strain. May want to reconstruct the mutations. Suppressors???? same as CL076 |
no | Box1, I1 | E. coli K-12 | |||
| 074 | 3/15/2002 | HL930 | J Courcelle | transduction with P1 from HL1058 | dut-21::cat | l-, thyA36, deoC2, IN(rrnD-rrnE)1, dut-21::cat, rph? see J Bact 175, 3401-3407 (1993) Not a great grower???? I worry about this strain. May want to reconstruct the mutations. Suppressors???? |
no | Box1, I2 | E. coli K-12 | ||||
| 075 | 3/15/2002 | HL928 | J Courcelle | transduction with P1 from HL1058 | dut-21::cat | l-, thyA36, deoC2, IN(rrnD-rrnE)1,ung-152::Tn10, dut-21::cat, rph? see J Bact 175, 3401-3407 (1993) Not a great grower???? I worry about this strain. May want to reconstruct the mutations. Suppressors???? same as CL063 |
no | Box1, I3 | E. coli K-12 | ||||
| 076 | 3/15/2002 | HL928 | HL930 | J Courcelle | transduction with P1 from BW560 (=HL670) | TetR (ung-) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, ung-152::Tn10, rph? see J Bact 175, 3401-3407 (1993) Not a great grower???? I worry about this strain. May want to reconstruct the mutations. Suppressors???? same as CL073 |
no | Box1, I4 | E. coli K-12 | |||
| 077 | 3/15/2002 | HR838 HL1033 | JCC via Rosenberg lab | DxonA::Cat His+ | thr-1, ara-14, leuB6, D(gpt-proA)62, lacY1, tsx-33, galK2, l-, rac-, rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, DxonA::cat300 (xonA = sbcB) Genetics 142, 333-339 (1996); |
no | Box1, I5 | E. coli K-12 | |||||
| 078 | 3/15/2002 | M15[pREP4] | J Courcelle | transformation amp selection | ac, ara, gal, mtl, (recA+) M15 is the name of a lacZ deletion (J Mol Biol 8, 427-430 (1964); not sure whether the lac- mutation in this strain is D(lacZ)15. Genotype above is given in QIAexpressionist (1992). pREP4 (carries lacI; results in high levels of lac repressor protein; compatible with plasmids with ColE1 origin; use 25 µg/ml kanamycin for selection) pMALO11 is pMALp2 with the recO gene cloned into the polylinker. It complements a recO mutant for UV snesitivity. should be the same as CL071 but different isolate. |
pMALO11 | no | no | Box1, I6 | E. coli K-12 | |||
| 079 | 3/15/2002 | DH5alpha | J Courcelle via Ann Britt | transformation amp selection | pAT6-4phr | Ø80dlacZDM15, D(lacZYA-argF)U169, recA1, endA1, hsdR17 (rk- mk+), supE44, l-, thi-1, gyrA, relA1, phoA pAT6-4phr is a plasmid that contains a 6-4 photolyase from a plant which I cant rememer right now. It should be easily found in some of Ann Britts older papers. She sent us the plasmid should be same as CL070 but different isolate |
no | Box1, I7 | E. coli K-12 | ||||
| 080 | 3/15/2002 | M15[pREP4] | J Courcelle | transformation amp selection | ac, ara, gal, mtl, (recA+) M15 is the name of a lacZ deletion (J Mol Biol 8, 427-430 (1964); not sure whether the lac- mutation in this strain is D(lacZ)15. Genotype above is given in QIAexpressionist (1992). pREP4 (carries lacI; results in high levels of lac repressor protein; compatible with plasmids with ColE1 origin; use 25 µg/ml kanamycin for selection) pMALR4 is pMALp2 with the recR gene cloned into the polylinker. It complements a recR mutant for UV snesitivity. should be the same as CL046 but different isolate. |
pMALR4 | no | no | Box1, I8 | E. coli K-12 | |||
| 101 | 7/25/2002 | AB1157, HL36 | J Courcelle | thr-1, ara-14, leuB6, D(gpt-proA)62, lacY1, tsx-33, supE44, galK2, l-, rac-, hisG4(Oc), rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, (see J Bact 177, 7261-7264 (1995) for info about pro deletion) Bachmann, 1987 |
no | no | Box2, A1 | E. coli K-12 | |||||
| 102 | 7/25/2002 | W3110 | J Courcelle | l-, IN(rrnD-rrnE)1 | no | no | Box2, A2 | E. coli K-12 | |||||
| 103 | 7/25/2002 | MG1655 | J Courcelle | P1 transduction | lexA1:catR | l-, rph-1, lexA1:catR lexA1 is ~30-50% cotransducible with catR) lexA1 P1 provided by Jennifer Halliday |
no | no | Box2, A3 | E. coli K-12 | |||
| 104 | 7/25/2002 | MG1655 | J Courcelle | P1 transduction | lexA1:catR | l-, rph-1, lexA1:catR lexA1 is ~30-50% cotransducible with catR) lexA1 P1 provided by Jennifer Halliday should be the same as CL103 but different isolate |
no | no | Box2, A4 | E. coli K-12 | |||
| 105 | 7/25/2002 | MG1655 | J Courcelle | Transduction with P1 from V1307 (=HL932) | recB21, recC22, argA81::Tn10 | l-, rph-1, recB21, recC22, argA81::Tn10 |
no | Tn10 tetR | Box2, A5 | E. coli K-12 | |||
| 106 | 7/25/2002 | MG1655 | J Courcelle | P1 transduction from HL931 (V220) | recD1011, argA81::Tn10 | l-, rph-1, recD1011, argA81::Tn10 | no | Tn10 tetR | Box2, A6 | E. coli K-12 | |||
| 107 | 7/25/2002 | MG1655 | J Courcelle | P1 transduction from HL931 (V220) | recD1011, argA81::Tn10 | l-, rph-1, recD1011, argA81::Tn10 should be the same as C106 but different isolate |
no | Tn10 tetR | Box2, A7 | E. coli K-12 | |||
| 108 | 7/25/2002 | MG1655 | J Courcelle | P1 transduction from HL556 | recF332::Tn3 |
l-, rph-1, recF332::Tn3 may also be tnaA300::Tn10 frm HL556 during the transduction did not check this |
no | Tn3 ampR | Box2, A8 | E. coli K-12 | |||
| 109 | 7/25/2002 | MG1655 | J Courcelle | P1 transduction from HL556 | recF332::Tn3 |
l-, rph-1, recF332::Tn3 may also be tnaA300::Tn10 frm HL556 during the transduction did not check this should be the same as C108 but different isolate |
no | Tn3 ampR | Box2, A9 | E. coli K-12 | |||
| 110 | 7/25/2002 | GW2100 | AB1157 | J Courcelle via Brad Smith (MIT) | Tn5 (kan); |
umuC122::Tn5 (kan); thr-1, ara-14, leuB6, D(gpt-proA)62, lacY1, tsx-33, supE44, galK2, l-, rac-, hisG4(Oc), rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, (see J Bact 177, 7261-7264 (1995) for info about pro deletion) J Mol Biol 164, 175-192 |
no | Box2, B1 | E. coli K-12 | ||||
| 111 | 7/25/2002 | No info | J Courcelle | No info on the background or plasmid. need to look this up |
pKY137 | no | Box2, B2 | E. coli K-12 | |||||
| 112 | 7/25/2002 | AM207 | J Courcelle | P1 transduction from HL931 (V220) | recD1011, argA81::Tn10 |
thr-1, ara-14, leuB6, D(gpt-proA)62, lacY1, tsx-33, supE44, galK2, l-, rac-, hisG4(Oc), rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, recR252::Tn10-9, recD1011, argA81::Tn10 | no | no | Tn10-9kan Tn10tet | Box2, B3 | E. coli K-12 | ||
| 113 | 7/25/2002 | AFT228 NK2992 | J Courcelle via Gerry Smith (FHCRC) | argA81::Tn10 |
arg81::Tn10, l- J Bact 155, 664-680 (1983); Genetics 126, 25-40 (1990) |
no | no | Tn10tet | Box2, B4 | E. coli K-12 | |||
| 114 | 7/25/2002 | CL002 | J Courcelle | P1 transduction from JC10289 | D(srlR- recA)306::Tn10 |
l-, thyA36, deoC2, IN(rrnD-rrnE)1, D(srlR- recA)306::Tn10, rph? see J Bact 175, 3401-3407 (1993) Transduction with P1 from JC10289 (=HL456) Mol Gen Genet 183, 497-504 (1981); should be the same as CL002 |
no | no | Tn10tet | Box2, B5 | E. coli K-12 | ||
| 115 | 7/26/2002 | CL003 | J Courcelle | P1 transduction from V1307 | recB21, recC22, argA81::Tn10 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recB21, recC22, argA81::Tn10, rph? see J Bact 175, 3401-3407 (1993) should be the same as CL003 |
no | no | Tn10tet | Box2, B6 | E. coli K-12 | ||
| 116 | 7/26/2002 | JC15359 HL816 | AB1157 | Steve Sandler via J Courcelle | TetR (recF) | thr-1, ara-14, leuB6, D(gpt-proA)62, lacY1, tsx-33, supE44, galK2, l-, rac-, hisG4(Oc), rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, recF349, tnaA300::Tn10 J Bact 176, 3661-3672 (1994); Genetics 135, 643-654 (1993) |
no | no | Tn10tet | Box2, B7 | E. coli K-12 | ||
| 117 | 7/26/2002 | JC15716 | Ab1157 | Steve Sandler via J Courcelle | P1 transduction from RDK1541 to AB1157 | KanR (recO) | thr-1, ara-14, leuB6, D(gpt-proA)62, lacY1, tsx-33, supE44, galK2, l-, rac-, hisG4(Oc), rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, recO1504::Tn5 J Bact 176, 3661-3672 (1994) |
no | no | Tn5kan | Box2, B8 | E. coli K-12 | |
| 118 | 7/26/2002 | JC12123 HL960 | Ab1157 | P Modrich via D J Crowley | transduction with P1 | recJ284::Tn10 | thr-1, ara-14, leuB6, D(gpt-proA)62, lacY1, tsx-33, supE44, galK2, l-, rac-, hisG4(Oc), rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, recJ284::Tn10 | no | no | Tn10tet | Box2, B9 | E. coli K-12 | |
| 119 | 7/26/2002 | V220 HL931 | J C Courcelle (from G. Smith) | recD1011, argA81::Tn10, recF143, hisG4, met, rpsL31, galK2, xyl-5, Drac, l- Proc Nat Acad Sci 81, 7850-7854 (1984); |
no | no | Tn10tet | Box2, C1 | E. coli K-12 | ||||
| 120 | 7/26/2002 | V1307 HL931 | J C Courcelle (from G. Smith) | recB21, recC22, sbcB, argA81::Tn10, thr-1, ara-14, leuB6, D(gpt-proA)62, lacY1, tsx-33, galK2, l-, rac-, hisG4(Oc), rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, Genetics 126, 25-40 (1990) |
no | no | Tn10tet | Box2, C2 | E. coli K-12 | ||||
| 121 | 7/26/2002 | DH108 | Ann Britt Maybe? | ? | pKY137 | no | Box2, C3 | E. coli K-12 | |||||
| 122 | 7/26/2002 | ? | Ann Britt Maybe? | ?, pKY137, p64AT | pKY137, p64AT | no | Box2, C4 | E. coli K-12 | |||||
| 123 | 7/26/2002 | AM207 | AB1157 | Steve Sandler via J Courcelle | P1 transduction from AM134 to AB1157 | recR252::Tn10-9 | thr-1, ara-14, leuB6, D(gpt-proA)62, lacY1, tsx-33, supE44, galK2, l-, rac-, hisG4(Oc), rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, recR252::Tn10-9 J Bact 176, 3661-3672 (1994); Mol Gen Genet 216, 503-510 (1989) |
no | Tn10-9kanR | Box2, C5 | E. coli K-12 | ||
| 124 | 7/26/2002 | W3110 | J Courcelle | P1 transduction from KD2250 | recQ1803::Tn3 | l-, IN(rrnD-rrnE)1, recQ1803::Tn3 | no | Tn3 ampR | Box2, C6 | E. coli K-12 | |||
| 125 | 7/26/2002 | W3110 | J Courcelle | P1 transduction from JC12123 | recJ284::Tn10 | l-, IN(rrnD-rrnE)1, recJ284::Tn10 | no | Tn10 | Box2, C7 | E. coli K-12 | |||
| 126 | 7/26/2002 | CL125 | J Courcelle | P1 transduction from AM207 | recR252::Tn10-9 |
l-, IN(rrnD-rrnE)1, recJ284::Tn10, recR252::Tn10-9 |
no | Tn10-9 kanR | Box2, C8 | E. coli K-12 | |||
| 127 | 7/26/2002 | W3110 | J Courcelle | P1 transduction from AM207 | recR252::Tn10-9 |
l-, IN(rrnD-rrnE)1, recR252::Tn10-9 |
no | Tn10-9 kanR | Box2, C9 | E. coli K-12 | |||
| 128 | 7/26/2002 | W3110 | J Courcelle | P1 transduction from HL556 | recF332::Tn3 | l-, IN(rrnD-rrnE)1, recF332::Tn3 maybe tna::Tn10 also? |
no | Tn3 ampR | Box2, D1 | E. coli K-12 | |||
| 129 | 7/26/2002 | W3110 | J Courcelle | P1 transduction from V1307 | recB21, recC22, argA81::Tn10 | l-, IN(rrnD-rrnE)1, recB21, recC22, argA81::Tn10 | no | Tn10 tetR | Box2, D2 | E. coli K-12 | |||
| 130 | 7/26/2002 | CL135 | J Courcelle | P1 transduction from V220 | recD1011, argA81::Tn10 | l-, IN(rrnD-rrnE)1, Dtus::KanR, recD1011, argA81::Tn10 | no | Tn10 tetR maybe Tn5 | Box2, D3 | E. coli K-12 | |||
| 131 | 7/26/2002 | CL135 | J Courcelle | P1 transduction from V220 | recD1011, argA81::Tn10 | l-, IN(rrnD-rrnE)1, Dtus::KanR, recD1011, argA81::Tn10 should be the same as CL130 but different isolate |
no | Tn10 tetR maybe Tn5 | Box2, D4 | E. coli K-12 | |||
| 132 | 7/26/2002 | CL135 | J Courcelle | P1 transduction from V1307 | recB21, recC22, argA81::Tn10 | l-, IN(rrnD-rrnE)1, Dtus::KanR, recB21, recC22, argA81::Tn10 |
no | Tn10 tetR maybe Tn5 | Box2, D5 | E. coli K-12 | |||
| 133 | 7/26/2002 | CL135 | J Courcelle | P1 transduction from V1307 | recB21, recC22, argA81::Tn10 | l-, IN(rrnD-rrnE)1, Dtus::KanR, recB21, recC22, argA81::Tn10 should be the same as CL132 but different isolate |
no | Tn10 tetR maybe Tn5 | Box2, D6 | E. coli K-12 | |||
| 134 | 7/26/2002 | W3110 | J Courcelle | P1 transduction from V220 | recD1011, argA81::Tn10 | l-, IN(rrnD-rrnE)1, recD1011, argA81::Tn10 | no | Tn10 tetR | Box2, D7 | E. coli K-12 | |||
| 135 | 7/26/2002 | W3110 | J Courcelle | P1 transduction from TH457 | Dtus::KanR | l-, IN(rrnD-rrnE)1, Dtus::KanR | no | Tn10 tetR maybe Tn5 | Box2, D8 | E. coli K-12 | |||
| 136 | 7/26/2002 | CL135 | J Courcelle | P1 transduction from V220 | recD1011, argA81::Tn10 | l-, IN(rrnD-rrnE)1, Dtus::KanR, recD1011, argA81::Tn10 should be the same as CL130 but different isolate |
no | Tn10 tetR maybe Tn5 | Box2, D9 | E. coli K-12 | |||
| 137 | 7/6/2001 | HL920 | Justin Courcelle | transformation with pQER4 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recR252::Tn10-9, rph? see J Bact 175, 3401-3407 (1993) pQER4? has recR his tagged? |
pQER4 | no | Tn10-9 | Box1, E1 | E. coli K-12 | |||
| 138 | 7/6/2001 | DH5alpha | Justin Courcelle | transformation with pMALF11 | Ø80dlacZDM15, D(lacZYA-argF)U169, recA1, endA1, hsdR17 (rk- mk+), supE44, l-, thi-1, gyrA, relA1, phoA pMALF11 rcontains recF gene with Maltose binding protein tag |
pMALF11 | no | Box1, E2 | E. coli K-12 | ||||
| 139 | 7/6/2001 | M15 | Justin Courcelle | lac, ara, gal, mtl, (recA+) M15 is the name of a lacZ deletion (J Mol Biol 8, 427-430 (1964); not sure whether the lac- mutation in this strain is D(lacZ)15. Genotype above is given in QIAexpressionist (1992). pQEO4 rcontains recO gene with his tag (possibly Maltose binding protein tag instead) |
pQEO4 | no | Box1, E3 | E. coli K-12 | |||||
| 140 | 7/6/2001 | Justin Courcelle | recO1504::Tn5 not sure of other genetic mrkers in this strain. Sloppy bookkeeping pQEO4 contains recO gene with his tag |
pQEO4 | no | Box1, E4 | E. coli K-12 | ||||||
| 141 | 7/6/2001 | Justin Courcelle | transformation with pQE9 | recO1504::Tn5 not sure of other genetic mrkers in this strain. Sloppy bookkeeping pQE9 should be same background as CL140 |
pQE9 | no | Box1, E5 | E. coli K-12 | |||||
| 142 | 7/6/2001 | M15 | Justin Courcelle | transformation with pQE9 | lac, ara, gal, mtl, (recA+) M15 is the name of a lacZ deletion (J Mol Biol 8, 427-430 (1964); not sure whether the lac- mutation in this strain is D(lacZ)15. Genotype above is given in QIAexpressionist (1992). pQE9 |
pQE9 | no | Box1, E6 | E. coli K-12 | ||||
| 143 | 7/6/2001 | M15 | Justin Courcelle | transformation with pQE9 | lac, ara, gal, mtl, (recA+) M15 is the name of a lacZ deletion (J Mol Biol 8, 427-430 (1964); not sure whether the lac- mutation in this strain is D(lacZ)15. Genotype above is given in QIAexpressionist (1992). pO2 recO gene cloned into a his tag expression vector |
pO2 | no | Box1, E7 | E. coli K-12 | ||||
| 144 | 7/6/2001 | M15 | Justin Courcelle | transformation with pQE9 | recO1504::Tn5 not sure of other genetic mrkers in this strain. Sloppy bookkeeping pO2 recO gene cloned into a his tag expression vector |
pO2 | no | Box1, E8 | E. coli K-12 | ||||
| 145 | 7/6/2001 | M15 | Justin Courcelle | transformation with pMALF2 | lac, ara, gal, mtl, (recA+) M15 is the name of a lacZ deletion (J Mol Biol 8, 427-430 (1964); not sure whether the lac- mutation in this strain is D(lacZ)15. Genotype above is given in QIAexpressionist (1992). pMALF2 recF gene cloned into a maltose binding protein expression vector |
pMALF2 | no | Box1, E9 | E. coli K-12 | ||||
| 146 | 7/6/2001 | CL005 | Justin Courcelle | transformation with pQEF1 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recF349, tnaA300::Tn10, rph? see J Bact 175, 3401-3407 (1993) pQEF1 recF gene cloned into a his tag expression vector |
pQEF1 | no | Box1, F1 | E. coli K-12 | ||||
| 147 | 7/26/2002 | CL135 | J Courcelle | P1 transduction from V1307 | recB21, recC22, argA81::Tn10 | l-, IN(rrnD-rrnE)1, Dtus::KanR, recB21, recC22, argA81::Tn10 should be the same as CL132 |
no | Tn10 tetR maybe Tn5 | Box2, F2 | E. coli K-12 | |||
| 148 | 7/26/2002 | KD2250 | H Nakayama via J C Courcelle | l-, recQ1803::Tn3, (recQ1803::Tn3 was formerly called recQ107::Tn3) Mol Gen Genet 200, 266-271 (1985) |
no | Tn3 ampR | Box2, F3 | E. coli K-12 | |||||
| 149 | 7/26/2002 | JC15716 | J C Courcelle | transformation with pQEO4 | thr-1, ara-14, leuB6, D(gpt-proA)62, lacY1, tsx-33, supE44, galK2, l-, rac-, hisG4(Oc), rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, recO1504::Tn5 pQEO4 recO gene cloned into a his tag expression vector |
pQEO4 | no | Box2, F4 | E. coli K-12 | ||||
| 150 | 7/26/2002 | HL456 | A Ganesan from A J Clark | Tn10 excision | D(srlR-recA)306 TetR, uvrA6, thr-1, ara-14, leuB6, D(gpt-proA)62, lacY1, tsx-33, supE44, galK2, l-, rac-, hisG4(Oc), rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, thyA, deo Mol Gen Genet 183, 497-504 (1981); Genetics 93, 321-323 (1979) |
no | Box2, F5 | E. coli K-12 | |||||
| 151 | 7/6/2001 | M15 | Justin Courcelle | transformation with pMALF5 | lac, ara, gal, mtl, (recA+) M15 is the name of a lacZ deletion (J Mol Biol 8, 427-430 (1964); not sure whether the lac- mutation in this strain is D(lacZ)15. Genotype above is given in QIAexpressionist (1992). pMALF5 recF gene cloned into a maltose binding protein expression vector |
pMALF5 | no | Box1, F6 | E. coli K-12 | ||||
| 152 | 7/6/2001 | TH457 HL971 | J C Courcelle, from T M Hill | As of 5/97 all I know is that this is a KanR Dtus::Tn5 (not even sure it is Tn5 or just KanR). The reference above is not much help; it lists a PK457, but the info supplied with HL971 suggests it is a derivative of MG1655, while PK457 is definitely not. Mol Microbiol 18, 45-61 (1995) |
no | ?KanR (maybe Tn5) | Box1, F7 | E. coli K-12 | |||||
| 153 | 7/26/2002 | JC15716 | J C Courcelle | transformation with pQEO2 | thr-1, ara-14, leuB6, D(gpt-proA)62, lacY1, tsx-33, supE44, galK2, l-, rac-, hisG4(Oc), rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, recO1504::Tn5 pQEO2 recO gene cloned into a his tag expression vector |
pQEO2 | no | Box2, F8 | E. coli K-12 | ||||
| 154 | 7/26/2002 | CL001 | J C Courcelle | transformation with pQE9 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph pQE9 a his tag expression vector |
pQE9 | no | Box2, F9 | E. coli K-12 | ||||
| 155 | 7/6/2001 | HL922 | Justin Courcelle | transformation with pTD | pTD | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recB21, recC22, argA81::Tn10, rph? see J Bact 175, 3401-3407 (1993) no idea what pTD is. maybe a Tus expressor? |
pTD | no | Tn10 | Box1, G1 | E. coli K-12 | ||
| 156 | 7/6/2001 | HL1062 | C Sundberg rotation student with hanawalt | P1 transduction from ??? | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recQ, recA no info on alleles or how strain was constructed |
no | Box1, G2 | E. coli K-12 | |||||
| 157 | 7/6/2001 | HL923 | Justin Courcelle | transformation with pTD | pTD | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recD1011, argA81::Tn10, rph? see J Bact 175, 3401-3407 (1993) no idea what pTD is. maybe a Tus expressor? |
pTD | no | Tn10 | Box1, G3 | E. coli K-12 | ||
| 158 | 7/6/2001 | HL954 | HL205 | Justin Courcelle | transduction with P1 from HL816 | thr, leu, met, thyA, (deo?), tonA, dnaB266, recF349, tna300::Tn10 ***temp sens*** |
no | Tn10 | Box1, G4 | E. coli K-12 | |||
| 159 | 7/6/2001 | HL311, E486 | J. Wechsler | thr, leu, thi, thyA, deo, lac, rpsL, tonA, SuII+, dnaE486, (met?) **thermosensitive** Proc Nat Acad Sci 68, 3150-3153 (1971); Mol Gen Genet 113, 273-284 (1971) |
no | Tn10 | Box1, G5 | E. coli K-12 | |||||
| 160 | 7/26/2002 | W3110 | J Courcelle | P1 transduction from JC10289 | D(srlR- recA)306::Tn10 | l-, IN(rrnD-rrnE)1, D(srlR- recA)306::Tn10 |
no | Tn10 | Box2, G6 | E. coli K-12 | |||
| 161 | 7/6/2001 | HL953 | HL205 | Justin Courcelle | transduction with P1 from JC15359 | thr, leu, met, thyA, (deo?), tonA, dnaB266, recR252::Tn10-9 ***temp sens*** |
no | Tn10 | Box1, G7 | E. coli K-12 | |||
| 162 | 7/6/2001 | HL78, SR78 | A Ganesan | thyA, deo, recB21, thr-1, leuB6, D(gpt-proA)62, lacY1, tsx-33, supE44, galK2, l-, rac-, hisG4(Oc), rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, **Reisolated 7/95 by J C Courcelle; new stab and also frozen culture. |
no | no | Box1, G8 | E. coli K-12 | |||||
| 163 | 7/6/2001 | HL545 | P.K. Cooper | thyA, deo, recF143, argE3,his-4, leuB6, proA2, thr-1, ara-14, galK2, lacY1, mtl-1, xyl-5, thi-1, rpsL131, supE44, tsx-33 J. Bact. 157, 500 (1984) |
no | no | Box1, G9 | E. coli K-12 | |||||
| 164 | 7/6/2001 | RW82 | J C Courcelle from Brad Smith (MIT) | DumuDC595::cat, uvrA6, can't tell what the rest of the genotype is from papers given. Mutat Res 281, 221-225 (1992); Mol Gen Genet 156, 121-131 (1977); |
no | no | Box1, H1 | E. coli K-12 | |||||
| 165 | 7/6/2001 | HL919, CL005 | SR108 (=HL930) | Justin Courcelle | Transduction with P1 from JC15359 (HL816) | TetR (recF, tnaA) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recF349, tnaA300::Tn10, rph? see J Bact 175, 3401-3407 (1993) Proc Nat Acad Sci 81, 7850-7854 (1984) same as CL005 |
no | no | Tn10 | Box1, H2 | E. coli K-12 | |
| 166 | 7/6/2001 | HL954 CL158 | HL205 | Justin Courcelle | transduction with P1 from HL816 | thr, leu, met, thyA, (deo?), tonA, dnaB266, recF349, tna300::Tn10 ***temp sens*** same as CL158 |
no | Tn10 | Box1, H3 | E. coli K-12 | |||
| 167 | 7/6/2001 | CL001 | Justin Courcelle | transformation with pTD | pTD | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph? no idea what pTD is. maybe a Tus expressor? |
pTD | no | Tn10 | Box1, H4 | E. coli K-12 | ||
| 168 | 7/6/2001 | HL953 | HL205 | Justin Courcelle | transduction with P1 from JC15359 | thr, leu, met, thyA, (deo?), tonA, dnaB266, recR252::Tn10-9 ***temp sens*** same as CL161 |
no | Tn10 | Box1, H5 | E. coli K-12 | |||
| 169 | 7/6/2001 | HL968 CL015 | HL920 | Justin Courcelle | Transduction with P1 from HL931(V220) | recD::Tn10 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recR252::Tn10-9, recD1011, argA81::Tn10, rph? see J Bact 175, 3401-3407 (1993) NB: recD1011 was originally designated recB1011 Proc Nat Acad Sci 81, 7850-7854 (1984); same as CL015 |
no | no | Tn10 tetR, Tn10-9 KanR | Box1, H6 | E. coli K-12 | |
| 170 | 7/6/2001 | HL920 CL006 | SR108 (=HL930) | Justin Courcelle | Transduction with P1 from JC15359 (HL816) | KanR (recR) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recR252::Tn10-9, rph? see J Bact 175, 3401-3407 (1993) J Bact 176, 3661-3672 (1994); Proc Nat Acad Sci 81, 7850-7854 (1984) same as CL006 |
no | no | Tn10-9 | Box1, H7 | E. coli K-12 | |
| 171 | 3/15/2002 | HL205 CRT266 PCH5 CL055 | Y. Hirota | thr, leu, met, thyA, (deo?), tonA, dnaB266 ***temp sens*** confirmed temp sens, thy- and requirement for amino acids (casamino acids) 8/94 Ann Inst Pasteur 110, 465 same as CL055 |
no | no | Box1, H8 | E. coli K-12 | |||||
| 172 | 7/6/2001 | HL1062 CL156 | C Sundberg rotation student with hanawalt | P1 transduction from ??? | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recQ, recA no info on alleles or how strain was constructed same as CL156 |
no | Box1, H9 | E. coli K-12 | |||||
| 173 | 3/15/2002 | HL205 CRT266 PCH5 CL055 | Y. Hirota | thr, leu, met, thyA, (deo?), tonA, dnaB266 ***temp sens*** confirmed temp sens, thy- and requirement for amino acids (casamino acids) 8/94 Ann Inst Pasteur 110, 465 same as CL055 |
no | no | Box1, I1 | E. coli K-12 | |||||
| 174 | 7/6/2001 | HL924 CL010 | SR108 (=HL930) | Justin Courcelle | Transduction with P1 from JC12123 | TetR (recJ) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recJ284::Tn10, rph? J Bact 157, 190-196 (1984) same as CL010 |
no | no | Tn10 (TetR) | Box1, I2 | E. coli K-12 | |
| 175 | 3/15/2002 | HL925 CL022 | HL930 | Justin Courcelle | Transduction with P1 from HL765 | TetR uvrC | l-, uvrC297::Tn10, thyA36, deoC2, IN(rrnD-rrnE)1, rph? see J Bact 175, 3401-3407 (1993) same as CL022 |
no | no | Tn10 (TetR) | Box1, I3 | E. coli K-12 | |
| 176 | 7/25/2002 | AM207 | J Courcelle | P1 transduction from (V1307) | recB21, recC22, argA81::Tn10 |
thr-1, ara-14, leuB6, D(gpt-proA)62, lacY1, tsx-33, supE44, galK2, l-, rac-, hisG4(Oc), rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, recR252::Tn10-9, recB21, recC22, argA81::Tn10 | no | no | Tn10-9kan Tn10tet | Box2, I4 | E. coli K-12 | ||
| 177 | 7/6/2001 | HL939 CL019 | HL930 | dj crowley | transduction with P1 from HL667 | mfd::kan | l-, thyA36, deoC2, IN(rrnD-rrnE)1, mfd::kan same as CL019 |
no | no | kanR | Box1, I5 | E. coli K-12 | |
| 178 | 7/25/2002 | MG1655 | J Courcelle | P1 transduction | lexA1:catR | l-, rph-1, lexA1:catR lexA1 is ~30-50% cotransducible with catR) lexA1 P1 provided by Jennifer Halliday same as CL103 |
no | no | Box2, I6 | E. coli K-12 | |||
| 179 | 3/15/2002 | CL058 | HL205 | J Courcelle | p1 transduction from HL817 |
KanR recO1504::tn5 | thr, leu, met, thyA, (deo?), tonA, dnaB266, recO1504::Tn5 ***temp sens*** same as CL058 |
no | Tn5 kanR | Box1, I7 | E. coli K-12 | ||
| 180 | 7/6/2001 | HL812, SR108, CL001 | W3110 | Justin Courcelle | trimethoprim low thy | thy- | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph (pyrimidine starvation)? see J Bact 175, 3401-3407 (1993) Should be the same as HL108 and HL812, but received from J C Courcelle as the direct parent of his strains HL919-925, and 928. Nature 224, 1164-1166 (1969) original same as CL001 |
no | no | no | Box1, I8 | E. coli K-12 | |
| 181 | 3/15/2002 | MG1655 CL043 | Courcelle via A.Khodurski | "wild type" (but l-) "It contains a frameshift mutation at the end of rph, causing low expression of the downstream gene pyrE and, in turn, a pyrimidine starvation phenotype. " used for genomic microarrays Science 277, 1453-1474 (1997); same as CL043 |
no | no | no | Box1, I9 | E. coli K-12 | ||||
| 501 | 7/3/2001 | HL930 | Justin Courcelle | P1 transduction | lexA1:catR | thyA36, deoC2, IN(rrnD-rrnE)1, rph (pyrimidine starvation)? see J Bact 175, 3401-3407 (1993),.lexA1(ind-), Tn??(catR) (lexA1 is ~30-50% cotransducible with catR) lexA1 P1 provided by Jennifer Halliday |
no | no | unknown | Box3, A1 | E. coli K-12 | ||
| 502 | 7/3/2001 | HL946 | Justin Courcelle | P1 transduction | lexA1:catR | thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3 lexA1(ind-):Tn??(catR) (lexA1 is ~30-50% cotransducible with catR) lexA1 P1 provided by Jennifer Halliday |
no | no | unknown | Box3, A2 | E. coli K-12 | ||
| 503 | 7/3/2001 | HL946 | Justin Courcelle | P1 transduction | lexA1:catR | thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3 lexA1(ind-):Tn??(catR) (lexA1 is ~30-50% cotransducible with catR) lexA1 P1 provided by Jennifer Halliday should be the same as CL502 but different isolate |
no | no | unknown | Box3, A3 | E. coli K-12 | ||
| 504 | 7/3/2001 | HL621 | DH5a | I Mellon via DJ Crowley | transformation | pZH-10 ampR | 80dlacZdelM15, del(lacZYA-argF)U169, recA1, endA1, hsdR17 (rk- mk+), supE44, l-, thi-1, gyrA, relA1, phoA pZH-10 = (pGEM-3Z + EcoRI-SstI frag (1 kb) of lacZ from pGA293) |
pZH-10 | no | no | Box3, A4 | E. coli K-12 | |
| 505 | 7/3/2001 | HL820 | DH5a | I Mellon via DJ Crowley | transformation | pRib1 ampR | 80dlacZdelM15, del(lacZYA-argF)U169, recA1, endA1, hsdR17 (rk- mk+), supE44, l-, thi-1, gyrA, relA1, phoA pRib 1 = (pGEM3Z with 1 kb SstI/SalI derived from E. coli ribosomal genes) |
pRib1 | no | no | Box3, A5 | E. coli K-12 | |
| 506 | 7/3/2001 | HL821 | DH5a | I Mellon via DJ Crowley | transformation | pRib2 ampR | Ø80dlacZdelM15, del(lacZYA-argF)U169, recA1, endA1, hsdR17 (rk- mk+), supE44, l-, thi-1, gyrA, relA1, phoA pRib 2 = (Bluescript KS vector with 520 bp BamHI/SstII fragment derived from E. coli rrnB) |
pRib2 | no | no | Box3, A6 | E. coli K-12 | |
| 507 | 7/3/2001 | CL001 | J Courcelle | transformation with pGEM-T Easy ampR | pGem-T Easy AmpR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph (pyrimidine starvation)? see J Bact 175, 3401-3407 (1993) pGem-T easy ampR |
pGem-T Easy | no | no | Box3, A7 | E. coli K-12 | ||
| 508 | 7/3/2001 | CL002 | J Courcelle | transformation with pGEM-T Easy ampR | pGem-T Easy AmpR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, D(srlR- recA)306::Tn10, rph? see J Bact 175, 3401-3407 (1993) pGem-T easy ampR |
pGem-T Easy | no | Tn10 tetR | Box3, A8 | E. coli K-12 | ||
| 509 | 7/3/2001 | JM109 | A Rooney from Promega | Bacterial Strain JM109 is a useful host for transformation of pGEM® Vectors and for production of single-stranded DNA from M13 or phagemid vectors. The strain grows well and is transformed efficiently by a variety of methods. Because JM109 is recA– and lacks the E. coli K restriction system, undesirable restriction of cloned DNA and recombination with host chromosomal DNA are prevented. The endonuclease A– mutation leads to an improved yield and quality of isolated plasmid DNA. JM109 is deficient in b-galactosidase activity due to deletions in both genomic and episomal copies of the lacZ gene. The deletion in the episomal (F' factor) copy of the lacZ gene (lacZDM15) can be complemented by addition of a functional a-peptide encoded by a pGEM®-Z or pGEM®-Zf Vector. If complementation does not occur, bacterial colonies are white. To maintain the F', JM109 should be grown on minimal (M-9) media supplemented with 1mM thiamine. Features * Reliable: Grows well and is transformed efficiently. * Versatile: Useful for cloning, single-stranded DNA production, and blue/white screening. * High Yields of Plasmid DNA: The endonuclease A– mutation improves yield and quality of isolated plasmid DNA. Applications * Blue/white color screening of pGEM®-Z and pGEM®-Zf(+/–) Vectors in the presence of X-Gal and IPTG. * Production of cloned inserts as single-stranded DNA from pGEM®-Zf Vectors or M13. |
no | no | no | Box3, A9 | E. coli K-12 | ||||
| 510 | 7/3/2001 | JM109 | A Rooney from Promega | transformation with pGEM-T Easy ampR | pGem-T Easy AmpR | Bacterial Strain JM109 is a useful host for transformation of pGEM® Vectors and for production of single-stranded DNA from M13 or phagemid vectors. The strain grows well and is transformed efficiently by a variety of methods. Because JM109 is recA– and lacks the E. coli K restriction system, undesirable restriction of cloned DNA and recombination with host chromosomal DNA are prevented. The endonuclease A– mutation leads to an improved yield and quality of isolated plasmid DNA. JM109 is deficient in b-galactosidase activity due to deletions in both genomic and episomal copies of the lacZ gene. The deletion in the episomal (F' factor) copy of the lacZ gene (lacZDM15) can be complemented by addition of a functional a-peptide encoded by a pGEM®-Z or pGEM®-Zf Vector. If complementation does not occur, bacterial colonies are white. To maintain the F', JM109 should be grown on minimal (M-9) media supplemented with 1mM thiamine. Features * Reliable: Grows well and is transformed efficiently. * Versatile: Useful for cloning, single-stranded DNA production, and blue/white screening. * High Yields of Plasmid DNA: The endonuclease A– mutation improves yield and quality of isolated plasmid DNA. Applications * Blue/white color screening of pGEM®-Z and pGEM®-Zf(+/–) Vectors in the presence of X-Gal and IPTG. * Production of cloned inserts as single-stranded DNA from pGEM®-Zf Vectors or M13. |
pGem-T Easy | no | no | Box3, B1 | E. coli K-12 | ||
| 511 | 10/24/2001 | RDK1541 SMR733 | RDK1041 | Kolodner via S Rosenberg | recO::Tn5 kanR | SuII+ recO1504::Tn5 thi-1, his-4, proA2 argE3 thr-1 leuB6 ara-14 lacY1 galK2 xyl-5 mtl-1 kdgK51 tsx-33 rpsL31 | no | no | Tn5 kanR | Box3, B2 | E. coli K-12 | ||
| 512 | 10/24/2001 | SR2210 | KSmith, ColiGenStockCtr | eda-51::Tn10 | thr-1 araC14 leuB6 DE(gpt-proA)62 lacY1 tsx-33 qsr'-0 glnV44(AS) galK2(Oc) LAM- Rac-0 eda-51::Tn10 ruvA200 hisG4(Oc) rfbD1 mgl-51 rpoS396(Am) rpsL31(strR) kdgK51 xylA5 mtl-1 argE3(Oc) thi-1 Sargentini, N.J., K.C. Smith 1989. Role of RuvAB genes in UV- and gamma-radiation and chemical mutagenesis in Escherichia coli. Mutat.Res. 215:115-129 |
no | no | Tn10 tetR | Box3, B3 | E. coli K-12 | |||
| 513 | 10/24/2001 | N4453 | RLloyd, ColiGenStockCtr | ruvC67::cat | thr-1 araC14 leuB6 DE(gpt-proA)62 lacY1 tsx-33 qsr'-0 glnV44(AS) galK2(Oc) LAM- Rac-0 ruvC67::cat hisG4(Oc) rfbD1 mgl-51 rpoS396(Am) rpsL31(strR) kdgK51 xylA5 mtl-1 argE3(Oc) thi-1 {\it ruvC} allele described in Seigneur et al. 1998, strain in Lloyd lab publication in press |
no | no | ? CamR | Box3, B4 | E. coli K-12 | |||
| 514 | 10/24/2001 | N4452 | RLloyd, ColiGenStockCtr | recG265::cat | thr-1 araC14 leuB6 DE(gpt-proA)62 lacY1 tsx-33 qsr'-0 glnV44(AS) galK2(Oc) LAM- Rac-0 recG265::cat hisG4(Oc) rfbD1 mgl-51 rpoS396(Am) rpsL31(strR) kdgK51 xylA5 mtl-1 argE3(Oc) thi-1 strain in Lloyd lab publication in press 2001 |
no | no | ? CamR | Box3, B5 | E. coli K-12 | |||
| 515 | 10/24/2001 | RDK2655 N2731 SMR600 | Kolodner via S Rosenberg | recG258::Tn10miniKn | recG258::Tn10miniKn others??? |
no | no | Tn10miniKan | Box3, B6 | E. coli K-12 | |||
| 516 | 10/24/2001 | RDK2615 CS85 SMR601 | Kolodner via S Rosenberg | eda-51::Tn10 tetR | ruvC53 eda-51::Tn10 thi-1 rpsL31 kdgK51 tsx-33 his-4 argE3 leuB6 thr-1 galK2 lacY1 ara-14 xyl5 mtl-1 |
no | no | Tn10 tetR | Box3, B7 | E. coli K-12 | |||
| 517 | 10/24/2001 | RDK2641 B713 SMR603 | Kolodner via S Rosenberg | ruvA59::Tn10 tetR | ruvA59::Tn10 recB21 recC22 sbcB15 sbcC201 hsdrK-mK+? according to Lloyd, this is a polar mutation (ruvA200 is the only one that’s not) |
no | no | Tn10 tetR | Box3, B8 | E. coli K-12 | |||
| 518 | 10/30/2001 | HL433+pBr322 | HL433 | Ann Ganesan | recA13, thr-1, ara-14, leuB6, del(gpt-proA)62, lacY1, tsx-33, supE44, galK2, l-, rac-, hisG4 (Oc), rfbD1, mgl-51, rpsL31, kdgK51, xyl-5, mtl-1, argE3, thi-1, thyA16, deoB11 New England Biolabs or BRL catalogs for map |
pBR322 (TetR AmpR) | no | Box3, B9 | E. coli K-12 | ||||
| 519 | 10/30/2001 | TP507 | AB1157 | Anthony Poteete | F- thr-1 ara-14 leuB6 Del(gpt-proA)62 lacY1 tsx-33 supE44 galK2 l- rac- hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 qsr- Del (recC ptr recB recD)::P tac -gam-red-pae-cI822 K.C. Murphy et al. (2000) Gene 246 : 321–330 |
no | Box3, C1 | E. coli K-12 | |||||
| 520 | 10/30/2001 | TP538 | TP507 | Anthony Poteete | transformation tetR | recG6200(sub tet857 for cdn4–691) | F- thr-1 ara-14 leuB6 Del(gpt-proA)62 lacY1 tsx-33 supE44 galK2 l- rac- hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 qsr- Del (recC ptr recB recD)::P tac -gam-red-pae-cI822 recG6200 (sub tet857 for cdn4–691) K.C. Murphy et al. (2000) Gene 246 : 321–330 |
no | Box3, C2 | E. coli K-12 | |||
| 521 | 10/30/2001 | TP539 | TP507 | Anthony Poteete | transformation kanR | recG6201(sub kan858 for cdn4–691) | F- thr-1 ara-14 leuB6 Del(gpt-proA)62 lacY1 tsx-33 supE44 galK2 l- rac- hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 qsr- Del (recC ptr recB recD)::P tac -gam-red-pae-cI822 recG6201(sub kan858 for cdn4–691) K.C. Murphy et al. (2000) Gene 246 : 321–330 |
no | Box3, C3 | E. coli K-12 | |||
| 522 | 10/30/2001 | TP540 | TP507 | Anthony Poteete | transformation tetR | ruvAB6203 (sub tet857 for [ruvAcdn4-ruvBcdn334] ) | F- thr-1 ara-14 leuB6 Del(gpt-proA)62 lacY1 tsx-33 supE44 galK2 l- rac- hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 qsr- Del (recC ptr recB recD)::P tac -gam-red-pae-cI822 ruvAB6203 (sub tet857 for [ruvAcdn4-ruvBcdn334] ) K.C. Murphy et al. (2000) Gene 246 : 321–330 |
no | Box3, C4 | E. coli K-12 | |||
| 523 | 10/30/2001 | TP541 | TP507 | Anthony Poteete | transformation kanR | ruvAB6204(sub kan858 for [ruvAcdn4-ruvBcdn334] ) | F- thr-1 ara-14 leuB6 Del(gpt-proA)62 lacY1 tsx-33 supE44 galK2 l- rac- hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 qsr- Del (recC ptr recB recD)::P tac -gam-red-pae-cI822 ruvAB6204(sub kan858 for [ruvAcdn4-ruvBcdn334] ) K.C. Murphy et al. (2000) Gene 246 : 321–330 |
no | Box3, C5 | E. coli K-12 | |||
| 524 | 10/30/2001 | TP577 | TP554 | Anthony Poteete | transformation tetR | recG6202 recF6206 (sub tet857 for cdn4–295) | F- thr-1 ara-14 leuB6 Del(gpt-proA)62 lacY1 tsx-33 supE44 galK2 l- rac- hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 qsr- Del (recC ptr recB recD)::P tac -gam-red-pae-cI822 recG6202 recF6206 (sub tet857 for cdn4–295) recG is unlinked to any marker K.C. Murphy et al. (2000) Gene 246 : 321–330 |
no | Box3, C6 | E. coli K-12 | |||
| 525 | 10/30/2001 | TP605 | KM37 | Anthony Poteete | transformation tetR | sulA6209 (sub tet857 for cdn9–153) | F- thr-1 ara-14 leuB6 Del(gpt-proA)62 lacY1 tsx-33 supE44 galK2 l- rac- hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 qsr- Del (recC ptr recB recD)::P tac -gam-red-pae-cI822 sulA6209 (sub tet857 for cdn9–153) K.C. Murphy et al. (2000) Gene 246 : 321–330 |
no | Box3, C7 | E. coli K-12 | |||
| 526 | 10/30/2001 | TP638 | TP507 | Anthony Poteete | transformation tetR | recQ6216 (sub tet857 for cdn19–606) | F- thr-1 ara-14 leuB6 Del(gpt-proA)62 lacY1 tsx-33 supE44 galK2 l- rac- hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 qsr- Del (recC ptr recB recD)::P tac -gam-red-pae-cI822 recQ6216 (sub tet857 for cdn19–606) K.C. Murphy et al. (2000) Gene 246 : 321–330 |
no | Box3, C8 | E. coli K-12 | |||
| 527 | 10/30/2001 | TP641 | TP612 | Anthony Poteete | transformation tetRkanR | recO6218 (sub tet857 for cdn3–240) | F- thr-1 ara-14 leuB6 Del(gpt-proA)62 lacY1 tsx-33 supE44 galK2 l- rac- hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 qsr- Del (recC ptr recB recD)::P tac -gam-red-pae-cI822 galK::Ptac-nin-kan878 recO6218 (sub tet857 for cdn3–240) K.C. Murphy et al. (2000) Gene 246 : 321–330 |
no | Box3, C9 | E. coli K-12 | |||
| 528 | 10/30/2001 | TP647 | TP507 | Anthony Poteete | transformation camR | recR6212 (sub cat883 for cdn4–182) | F- thr-1 ara-14 leuB6 Del(gpt-proA)62 lacY1 tsx-33 supE44 galK2 l- rac- hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 qsr- Del (recC ptr recB recD)::P tac -gam-red-pae-cI822 recR6212 (sub cat883 for cdn4–182) K.C. Murphy et al. (2000) Gene 246 : 321–330 |
no | Box3, D1 | E. coli K-12 | |||
| 529 | 10/30/2001 | TP648 | TP507 | Anthony Poteete | transformation camR | recQ6215 (sub cat883 for cdn19–606) | F- thr-1 ara-14 leuB6 Del(gpt-proA)62 lacY1 tsx-33 supE44 galK2 l- rac- hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 qsr- Del (recC ptr recB recD)::P tac -gam-red-pae-cI822 recQ6215 (sub cat883 for cdn19–606) K.C. Murphy et al. (2000) Gene 246 : 321–330 |
no | Box3, D2 | E. coli K-12 | |||
| 530 | 12/30/2001 | CL001 | Justin Courcelle | transformation | pBr322 ampRtetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph (pyrimidine starvation)? see J Bact 175, 3401-3407 (1993) Should be the same as HL108 and HL812, but received from J C Courcelle as the direct parent of his strains HL919-925, and 928. Nature 224, 1164-1166 (1969) original pBr322 ampR tetR |
pBr322 ampRtetR | no | no | Box3, D3 | E. coli K-12 | ||
| 531 | 12/30/2001 | CL002 | Justin Courcelle | transformation | pBr322 ampRtetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, D(srlR- recA)306::Tn10, rph? see J Bact 175, 3401-3407 (1993) Transduction with P1 from JC10289 (=HL456) Mol Gen Genet 183, 497-504 (1981); pBr322 ampR tetR |
pBr322 ampRtetR | no | Tn10 tetR | Box3, D4 | E. coli K-12 | ||
| 532 | 3/14/2002 | CL001 | Justin Courcelle | p1 transduction from CL517 |
ruvA59::Tn10 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph (pyrimidine starvation)? see J Bact 175, 3401-3407 (1993) ruvA59::Tn10 according to Lloyd, this is a polar mutation (ruvA200 is the only one that’s not) |
no | Tn10 tetR | Box3, D5 | E. coli K-12 | |||
| 533 | 3/14/2002 | CL001 | Justin Courcelle | p1 transduction from CL517 |
ruvA59::Tn10 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph (pyrimidine starvation)? see J Bact 175, 3401-3407 (1993) ruvA59::Tn10 according to Lloyd, this is a polar mutation (ruvA200 is the only one that’s not) should be same as CL532 but different isolate |
no | Tn10 | Box3, D6 | E. coli K-12 | |||
| 534 | 3/14/2002 | CL022 | Justin Courcelle | transformation with pBr322 |
pBr322 ampR tetR | l-, uvrA::Tn10, thyA36, deoC2, IN(rrnD-rrnE)1, rph? see J Bact 175, 3401-3407 (1993) pBr322 |
pBr322 ampR tetR | no | no | Box3, D7 | E. coli K-12 | ||
| 535 | 3/14/2002 | CL532 | Justin Courcelle | transformation with pBr322 | pBr322 ampR tetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph (pyrimidine starvation)? see J Bact 175, 3401-3407 (1993) ruvA59::Tn10 pBr322 |
pBr322 | no | Tn10 tetR | Box3, D8 | E. coli K-12 | ||
| 536 | 3/14/2002 | CL010 | Justin Courcelle | Transformation with pBr322 | pBr322 ampR tetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recJ284::Tn10, rph? J Bact 157, 190-196 (1984) pBr322 |
pBr322 | no | Tn10 (TetR) | Box3, D9 | E. coli K-12 | ||
| 537 | 3/14/2002 | CL004 HL923 | Justin Courcelle | transformation with pBr322 | pBr322 ampR tetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recD1011, argA81::Tn10, rph? see J Bact 175, 3401-3407 (1993) NB: recD1011 was originally designated recB1011 pBr322 |
pBr322 | no | Tn10 | Box3, E1 | E. coli K-12 | ||
| 538 | 3/14/2002 | CL003 HL922 | Justin Courcelle | transformation with pBr322 | pBr322 ampR tetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recB21, recC22, argA81::Tn10, rph? see J Bact 175, 3401-3407 (1993) pBr322 |
pBr322 | no | Tn10 | Box3, E2 | E. coli K-12 | ||
| 539 | 3/14/2002 | JJC432 | Morton&Hubenthal via Susan Lovett | leuB6 hisG4 argE3 lacY1 galK2 ara-14 xyl-5 mtl-1 tsx-33 rpsL31 supE44 hsdR recD1901::Tn10 recA::cam from Bzymek M, Saveson CJ, Feschenko VV, Lovett ST. Slipped misalignment mechanisms of deletion formation: in vivo susceptibility to nucleases, Journal of Bacteriology (1999) 181:477 |
no | Tn10 and Cam | Box3, E3 | E. coli K-12 | |||||
| 540 | 3/14/2002 | CL009 HL944 | Justin Courcelle | transformation with pBr322 | pBr322 ampR tetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recQ1803::Tn3 pBr322 |
pBr322 | no | Tn3 | Box3, E4 | E. coli K-12 | ||
| 541 | 3/14/2002 | CL005 HL919 | Justin Courcelle | transformation with pBr322 | pBr322 ampR tetR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recF349, tnaA300::Tn10, rph? see J Bact 175, 3401-3407 (1993) Proc Nat Acad Sci 81, 7850-7854 (1984) pBr322 |
pBr322 | no | Tn10 | Box3, E5 | E. coli K-12 | ||
| 542 | 3/14/2002 | CL001 | Justin Courcelle | p1 transduction from CL539 | recA::camR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph (pyrimidine starvation)? see J Bact 175, 3401-3407 (1993) recA::cam |
no | Box3, E6 | E. coli K-12 | ||||
| 543 | 3/14/2002 | CL001 | Justin Courcelle | p1 transduction from CL007 | recF332::ampR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3 J Bact 181, 916-922 (1999); This strain should be the same as CL007.. except that CL007 is TetR also. So it carried in tnaA300::Tn10 frm HL556 during the transduction as well. I did this transduction to make sure the strain was Tet sensitive! |
no | Box3, E7 | E. coli K-12 | ||||
| 544 | 5/14/2002 | CL001 | Justin Courcelle | p1 transduction from CL528 | recR6212 (sub cat883 for cdn4–182) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recR6212 (sub cat883 for cdn4–182) |
no | Box3, E8 | E. coli K-12 | ||||
| 545 | 5/14/2002 | CL001 | Justin Courcelle | p1 transduction from CL528 | recR6212 (sub cat883 for cdn4–182) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recR6212 (sub cat883 for cdn4–182) should be same as CL544 but different isolate |
no | Box3, E9 | E. coli K-12 | ||||
| 546 | 3/14/2002 | CL007 | Justin Courcelle | p1 transduction from CL528 | recR6212 (sub cat883 for cdn4–182) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3, recR6212 (sub cat883 for cdn4–182) |
no | Box3, F1 | E. coli K-12 | ||||
| 547 | 3/14/2002 | CL007 | Justin Courcelle | p1 transduction from CL528 | recR6212 (sub cat883 for cdn4–182) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3, recR6212 (sub cat883 for cdn4–182) should be the same as CL546 but different isolate |
no | Box3, F2 | E. coli K-12 | ||||
| 548 | 5/27/2002 | MG1655mutS | MG1655 | Pilar Francino | ? | mutS:strR | mutS:str | Box3 F3 | E. coli K12 | ||||
| 549 | 5/27/2002 | SA977 | Pilar Francino | rifR? | Hfr | Box3 F4 | Samonella Enterica | ||||||
| 550 | 5/27/2002 | SA965 | Pilar Francino | rifR? | Hfr | Box3 F5 | Samonella Enterica | ||||||
| 551 | 5/27/2002 | PHX | Pilar Francino | rifR? | Hfr | Box3 F6 | E. coli K12 | ||||||
| 552 | 5/27/2002 | PK3 | Pilar Francino | rifR? | Hfr | Box3 F7 | E. coli K12 | ||||||
| 553 | 5/27/2002 | CL001 | Justin Courcelle | p1 transduction from CL527 | recO6218 (sub tet857 for cdn3–240) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recO6218 (sub tet857 for cdn3–240) | no | no | Box3, F8 | E. coli K-12 | |||
| 554 | 5/27/2002 | CL001 | Justin Courcelle | p1 transduction from CL527 | recO6218 (sub tet857 for cdn3–240) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recO6218 (sub tet857 for cdn3–240) should be the same as CL553 but different isolate |
no | no | Box3, F9 | E. coli K-12 | |||
| 555 | 5/27/2002 | CL001 | Justin Courcelle | p1 transduction from CL513 | ruvC67::cat | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, ruvC67::cat | no | no | Box3, G1 | E. coli K-12 | |||
| 556 | 5/27/2002 | CL001 | Justin Courcelle | p1 transduction from CL513 | ruvC67::cat | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, ruvC67::cat should be the same as CL555 but a different isolate |
no | no | Box3, G2 | E. coli K-12 | |||
| 557 | 5/27/2002 | CL001 | Justin Courcelle | p1 transduction from CL522 | ruvAB6203 (sub tet857 for [ruvAcdn4-ruvBcdn334] ) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, ruvAB6203 (sub tet857 for [ruvAcdn4-ruvBcdn334] ) |
no | no | Box3, G3 | E. coli K-12 | |||
| 558 | 5/27/2002 | CL001 | Justin Courcelle | p1 transduction from CL522 | ruvAB6203 (sub tet857 for [ruvAcdn4-ruvBcdn334] ) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, ruvAB6203 (sub tet857 for [ruvAcdn4-ruvBcdn334] ) should be the same as CL557 but different isolate |
no | no | Box3, G4 | E. coli K-12 | |||
| 559 | 5/27/2002 | CL001 | Justin Courcelle | p1 transduction from CL521 | recG6201(sub kan858 for cdn4–691) |
l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recG6201(sub kan858 for cdn4–691) |
no | no | Box3, G5 | E. coli K-12 | |||
| 560 | 5/27/2002 | CL001 | Justin Courcelle | p1 transduction from CL521 | recG6201(sub kan858 for cdn4–691) |
l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recG6201(sub kan858 for cdn4–691) should be the same as CL559 but different isolate |
no | no | Box3, G6 | E. coli K-12 | |||
| 561 | 5/27/2002 | CL532 | Justin Courcelle | p1 transduction from CL521 |
recG6201(sub kan858 for cdn4–691) |
l-, thyA36, deoC2, IN(rrnD-rrnE)1, ruvA59::Tn10, recG6201(sub kan858 for cdn4–691) |
no | Tn10 tetR | Box3, G7 | E. coli K-12 | |||
| 562 | 5/27/2002 | CL532 | Justin Courcelle | p1 transduction from CL521 |
recG6201(sub kan858 for cdn4–691) |
l-, thyA36, deoC2, IN(rrnD-rrnE)1, ruvA59::Tn10, recG6201(sub kan858 for cdn4–691) should be the same as CL561 but different isolate |
no | Tn10 tetR | Box3, G8 | E. coli K-12 | |||
| 563 | 5/27/2002 | CL553 | Justin Courcelle | transformation with pBr322 | amp tet | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recO6218 (sub tet857 for cdn3–240) pBr322 |
pBr322 | no | no | Box3, G9 | E. coli K-12 | ||
| 564 | 5/27/2002 | CL555 | Justin Courcelle | transformation with pBr322 | amp tet | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, ruvC67::cat pBr322 |
pBr322 | no | no | Box3, H1 | E. coli K-12 | ||
| 565 | 5/27/2002 | CL559 | Justin Courcelle | transformation with pBr322 | amp tet |
l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recG6201(sub kan858 for cdn4–691) pBr322 |
pBr322 | no | no | Box3, H2 | E. coli K-12 | ||
| 566 | 5/27/2002 | CL561 | Justin Courcelle | transformation with pBr322 | amp tet |
l-, thyA36, deoC2, IN(rrnD-rrnE)1, ruvA59::Tn10, recG6201(sub kan858 for cdn4–691) pBr322 |
pBr322 | no | Tn10 tetR | Box3, H3 | E. coli K-12 | ||
| 567 | 5/27/2002 | CL562 | Justin Courcelle | transformation with pBr322 | amp tet |
l-, thyA36, deoC2, IN(rrnD-rrnE)1, ruvA59::Tn10, recG6201(sub kan858 for cdn4–691) should be the same as CL566 but different isolate pBr322 |
pBr322 | no | Tn10 tetR | Box3, H4 | E. coli K-12 | ||
| 568 | 5/14/2002 | CL544 | Justin Courcelle | transformation with pBr322 | amp tet | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recR6212 (sub cat883 for cdn4–182) pBr322 |
pBr322 | no | Box3, H5 | E. coli K-12 | |||
| 569 | 6/24/2002 | CL544 | Kin-Hoe Chow | P1 transduction from CL527 | recO:TET | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recR6212 (sub cat883 for cdn4–182), recO6218 (sub tet857 for cdn3–240) |
no | Box3, H6 | E. coli K-12 | ||||
| 570 | 6/24/2002 | CL544 | Kin-Hoe Chow | P1 transduction from CL527 | recO:TET | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recR6212 (sub cat883 for cdn4–182), recO6218 (sub tet857 for cdn3–240) should be same as CL569 but diferent isolate |
no | Box3, H7 | E. coli K-12 | ||||
| 571 | 6/24/2002 | CL008 | Janet Donaldson | transformation with pBr322 | amp tet | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recG::Tn5 pBr322 |
pBr322 | no | Box3, H8 | E. coli K-12 | |||
| 572 | 6/24/2002 | CL569 | Kin-Hoe Chow | P1 transduction from CL007 | recF332::Tn3 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recR6212 (sub cat883 for cdn4–182), recO6218 (sub tet857 for cdn3–240), recF332::Tn3 |
no | Box3, H9 | E. coli K-12 | ||||
| 573 | 6/24/2002 | CL569 | Kin-Hoe Chow | P1 transduction from CL007 | recF332::Tn3 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recR6212 (sub cat883 for cdn4–182), recO6218 (sub tet857 for cdn3–240), recF332::Tn3 should be same as CL572 but different isolate |
no | Box3, I1 | E. coli K-12 | ||||
| 574 | 6/24/2002 | CL553 | Kin-Hoe Chow | P1 transduction from CL007 | recF332::Tn3 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recO6218 (sub tet857 for cdn3–240), recF332::Tn3 should be same as CL572 but different isolate |
no | Box3, I2 | E. coli K-12 | ||||
| 575 | 6/24/2002 | CL001 | Kin-Hoe Chow | P1 transduction from GW2100 | umuC::kan | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, umuC122::Tn5 (kan) |
no | Box3, I3 | E. coli K-12 | ||||
| 576 | 6/24/2002 | CL001 | Justin Courcelle | P1 transduction from GW2100 | umuC::kan | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, umuC122::Tn5 (kan) should be same as CL575 but made in different expt. |
no | Box3, I4 | E. coli K-12 | ||||
| 577 | 6/24/2002 | CL001 | Justin Courcelle | P1 transduction from CL516 | ruvC53eda-51::Tn10 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, ruvC53, eda-51::Tn10 | no | tn10 | Box3, I5 | E. coli K-12 | |||
| 578 | 6/24/2002 | CL001 | Justin Courcelle | P1 transduction from CL523 | ruvAB::kan | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, ruvAB6204(sub kan858 for [ruvAcdn4-ruvBcdn334] ) | no | Box3, I6 | E. coli K-12 | ||||
| 579 | 6/24/2002 | CL001 | Justin Courcelle | P1 transduction from CL524 | recF::tet | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF6206 (sub tet857 for cdn4–295) |
no | Box3, I7 | E. coli K-12 | ||||
| 580 | 6/24/2002 | CL001 | Justin Courcelle | P1 transduction from CL524 | recF::tet | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF6206 (sub tet857 for cdn4–295) should be same as CL579 but different isolate |
no | Box3, I8 | E. coli K-12 | ||||
| 581 | 6/24/2002 | CL001 | Justin Courcelle | P1 transduction from CL529 | recQ::cam | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recQ6215 (sub cat883 for cdn19–606) |
no | Box3, I9 | E. coli K-12 | ||||
| 582 | 6/24/2002 | CL001 | Justin Courcelle | P1 transduction from CL529 | recQ::cam | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recQ6215 (sub cat883 for cdn19–606) should be the same as CL581 but different isolate |
no | Box4, A1 | E. coli K-12 | ||||
| 583 | 6/24/2002 | CL579 | Justin Courcelle | transformation | pBR322 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF6206 (sub tet857 for cdn4–295) pBR322 |
pBR322 | no | Box3, I7 | E. coli K-12 | |||
| 584 | 6/24/2002 | CL001 | Justin Courcelle | P1 transduction from CL511 | recO1504::Tn5 kanR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recO1504::Tn5 should be the same as CL583 but different isolate |
no | Tn5 | Box4, A3 | E. coli K-12 | |||
| 585 | 6/24/2002 | CL584 | Justin Courcelle | transformation | pBR322 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recO1504::Tn5 should be the same as CL583 but different isolate pBR322 |
pBR322 | no | Tn5 | Box4, A3 | E. coli K-12 | ||
| 586 | 6/24/2002 | CL577 | Justin Courcelle | transformation | pBR322 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, ruvC53, eda-51::Tn10 pBR322 |
pBR322 | no | tn10 | Box3, I5 | E. coli K-12 | ||
| 587 | 6/24/2002 | CL576 | Justin Courcelle | transformation | pBR322 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, umuC122::Tn5 (kan) pBR322 |
pBR322 | no | Box3, I4 | E. coli K-12 | |||
| 588 | 7/15/2002 | CL007 | Kin-Hoe Chow | P1 transduction from CL511 | recO1504::Tn5 kanR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3 tnaA300::Tn10, recO1504::Tn5 |
no | Tn5 Tn10 | Box4, A9 | E. coli K-12 | |||
| 589 | 7/15/2002 | CL007 | Kin-Hoe Chow | P1 transduction from CL511 | recO1504::Tn5 kanR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3 tnaA300::Tn10, recO1504::Tn5 Should be same as CL588 but different isolate |
no | Tn5 Tn10 | Box4, B1 | E. coli K-12 | |||
| 590 | 7/15/2002 | CL546 | Kin-Hoe Chow | P1 transduction from CL511 | recO1504::Tn5 kanR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3, recR6212 (sub cat883 for cdn4–182), recO1504::Tn5 |
no | Tn5 Tn3 | Box4, B2 | E. coli K-12 | |||
| 591 | 7/15/2002 | CL546 | Kin-Hoe Chow | P1 transduction from CL511 | recO1504::Tn5 kanR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recF332::Tn3, recR6212 (sub cat883 for cdn4–182), recO1504::Tn5 Should be same as CL590 but different isolate |
no | Tn5 Tn3 | Box4, B3 | E. coli K-12 | |||
| 592 | 7/15/2002 | CL544 | Kin-Hoe Chow | P1 transduction from CL511 | recO1504::Tn5 kanR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recR6212 (sub cat883 for cdn4–182), recO1504::Tn5 |
no | Tn5 | Box4, B4 | E. coli K-12 | |||
| 593 | 7/15/2002 | CL544 | Kin-Hoe Chow | P1 transduction from CL511 | recO1504::Tn5 kanR | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recR6212 (sub cat883 for cdn4–182), recO1504::Tn5 Should be same as CL592 but different isolate |
no | Tn5 | Box4, B5 | E. coli K-12 | |||
| 594 | 7/15/2002 | CL010 | Kin-Hoe Chow | P1 transduction from CL529 | recQ6215 (sub cat883 for cdn19–606) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recJ284::Tn10, rph?, recQ6215 (sub cat883 for cdn19–606) |
no | Tn10 | Box4, B6 | E. coli K-12 | |||
| 595 | 7/15/2002 | CL010 | Kin-Hoe Chow | P1 transduction from CL529 | recQ6215 (sub cat883 for cdn19–606) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recJ284::Tn10, rph?, recQ6215 (sub cat883 for cdn19–606) Should be same as CL594 but different isolate |
no | Tn10 | Box4, B7 | E. coli K-12 | |||
| 596 | 7/15/2002 | CL575 | Kin-Hoe Chow | P1 transduction from CL529 | recQ6215 (sub cat883 for cdn19–606) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, umuC::kan, recQ6215 (sub cat883 for cdn19–606) |
no | Box4, B8 | E. coli K-12 | ||||
| 597 | 7/15/2002 | CL575 | Kin-Hoe Chow | P1 transduction from CL529 | recQ6215 (sub cat883 for cdn19–606) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, umuC::kan, recQ6215 (sub cat883 for cdn19–606) Should be same as CL596 but different isolate |
no | Box4, B9 | E. coli K-12 | ||||
| 598 | 7/25/2002 | CL575 | Kin-Hoe Chow | P1 transduction from CL010 | recJ284::Tn10 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, umuC::kan, recJ284::Tn10 |
no | Tn10 | Box4, C1 | E. coli K-12 | |||
| 599 | 7/25/2002 | CL575 | Kin-Hoe Chow | P1 transduction from CL010 | recJ284::Tn10 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, umuC::kan, recJ284::Tn10 should be the same as CL598 but different isolate |
no | Tn10 | Box4, C2 | E. coli K-12 | |||
| 600 | 7/25/2002 | CL575 | Kin-Hoe Chow | P1 transduction from CL007 | recF332:Tn3 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, umuC::kan, recF332::Tn3 maybe tnaA300::Tn10 too? |
no | Tn3 maybe Tn10 too | Box4, C3 | E. coli K-12 | |||
| 601 | 7/25/2002 | CL575 | Kin-Hoe Chow | P1 transduction from CL007 | recF332:Tn3 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, umuC::kan, recF332::Tn3 maybe tnaA300::Tn10 too? should be the same as CL600 but different isolate |
no | Tn3 maybe Tn10 too | Box4, C4 | E. coli K-12 | |||
| 602 | 7/25/2002 | CL575 | Kin-Hoe Chow | P1 transduction from CL528 | recR6212 (sub cat883 for cdn4–182) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, umuC::kan, recR6212 (sub cat883 for cdn4–182) |
no | Box4, C5 | E. coli K-12 | ||||
| 603 | 7/25/2002 | CL575 | Kin-Hoe Chow | P1 transduction from CL528 | recR6212 (sub cat883 for cdn4–182) | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, umuC::kan, recR6212 (sub cat883 for cdn4–182) should be the same as CL602 but different isolate |
no | Box4, C6 | E. coli K-12 | ||||
| 604 | 7/25/2002 | MGZ | MG1655 | J Courcelle via Robert Fuchs | MG1655 lacY::Tn10 mini tet, lacIi, del(lacZ)(M15) EMBO 19:6259 (Napolitano et.al.2000) |
no | Tn10 | Box4, C7 | E. coli K-12 | ||||
| 605 | 7/25/2002 | MGZdinB | MGZ | J Courcelle via Robert Fuchs | MG1655 lacY::Tn10 mini tet, lacIi, del(lacZ)(M15), del(dinB)::Kan EMBO 19:6259 (Napolitano et.al.2000) |
no | Tn10 | Box4, C8 | E. coli K-12 | ||||
| 606 | 7/25/2002 | MGZpolB | MGZ | J Courcelle via Robert Fuchs | MG1655 lacY::Tn10 mini tet, lacIi, del(lacZ)(M15), del(polB)::omega spc EMBO 19:6259 (Napolitano et.al.2000) |
no | Tn10 | Box4, C9 | E. coli K-12 | ||||
| 607 | 7/25/2002 | MGZdinBpolB | MGZ | J Courcelle via Robert Fuchs | MG1655 lacY::Tn10 mini tet, lacIi, del(lacZ)(M15), del(polB)::omega spc, del(dinB)::Kan EMBO 19:6259 (Napolitano et.al.2000) |
no | Tn10, omega | Box4, D1 | E. coli K-12 | ||||
| 608 | 8/1/2002 | MG1655 | Charmain Courcelle | Transduction with P1 from V1307 (=HL932) | recB21, recC22, argA81::Tn10 | l-, rph-1, recB21, recC22, argA81::Tn10 |
no | Tn10 tetR | Box4, D2 | E. coli K-12 | |||
| 609 | 8/1/2002 | MG1655 | Charmain Courcelle | Transduction with P1 from V1307 (=HL932) | recB21, recC22, argA81::Tn10 | l-, rph-1, recB21, recC22, argA81::Tn10 should be the same as CL608 but different isolate |
no | Tn10 tetR | Box4, D3 | E. coli K-12 | |||
| 610 | 8/1/2002 | MG1655 | Charmain Courcelle | P1 transduction from HL931 (V220) | recD1011, argA81::Tn10 | l-, rph-1, recD1011, argA81::Tn10 | no | Tn10 tetR | Box2, D4 | E. coli K-12 | |||
| 611 | 8/1/2002 | MG1655 | Charmain Courcelle | P1 transduction from HL931 (V220) | recD1011, argA81::Tn10 | l-, rph-1, recD1011, argA81::Tn10 should be the same as CL610 but different isolate |
no | Tn10 tetR | Box2, D5 | E. coli K-12 | |||
| 612 | 8/1/2002 | MG1655 | Charmain Courcelle | P1 transduction from TH457 | tus:kan | l-, rph-1, tus::kan |
no | Tn5 maybe but unsure | Box2, D6 | E. coli K-12 | |||
| 613 | 8/1/2002 | MG1655 | Charmain Courcelle | P1 transduction from TH457 | tus:kan | l-, rph-1, tus::kan should be the same as CL612 but different isolate |
no | Tn5 maybe but unsure | Box2, D7 | E. coli K-12 | |||
| 614 | 8/1/2002 | CL001 | Charmain Courcelle | P1 transduction from TH457 | tus:kan | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph, tus::kan |
no | Tn5 maybe but unsure | Box2, D8 | E. coli K-12 | |||
| 615 | 8/1/2002 | CL001 | Charmain Courcelle | P1 transduction from TH457 | tus:kan | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph, tus::kan should be the same as CL614 but different isolate |
no | Tn5 maybe but unsure | Box2, D9 | E. coli K-12 | |||
| 616 | 8/1/2002 | CL614 | Charmain Courcelle | P1 transduction from CL003 | recB21, recC22, argA81::Tn10 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph, tus::kan, recB21, recC22, argA81::Tn10 |
no | Tn10 tetR | Box2, E1 | E. coli K-12 | |||
| 617 | 8/1/2002 | CL614 | Charmain Courcelle | P1 transduction from CL003 | recB21, recC22, argA81::Tn10 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph, tus::kan, recB21, recC22, argA81::Tn10 should be the same as CL616 but different isolate |
no | Tn10 tetR | Box2, E2 | E. coli K-12 | |||
| 618 | 8/1/2002 | CL004 | Charmain Courcelle | P1 transduction from CL614 | tus::kan | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph, tus::kan, recD1011, argA81::Tn10 |
no | tn5? maybe | Box2, E3 | E. coli K-12 | |||
| 619 | 8/1/2002 | CL004 | Charmain Courcelle | P1 transduction from CL614 | tus::kan | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph, tus::kan, recD1011, argA81::Tn10 should be the same as CL618 but different isolate |
no | tn5? maybe | Box2, E4 | E. coli K-12 | |||
| 620 | 8/1/2002 | CL612 | Charmain Courcelle | Transduction with P1 from V1307 (=HL932) | recB21, recC22, argA81::Tn10 | l-, rph-1, tus::kan, recB21, recC22, argA81::Tn10 ***not made yet |
no | Box2, E5 | E. coli K-12 | ||||
| 621 | 8/1/2002 | CL612 | Charmain Courcelle | Transduction with P1 from V1307 (=HL932) | recB21, recC22, argA81::Tn10 | l-, rph-1, tus::kan, recB21, recC22, argA81::Tn10 ***not made yet |
no | Box2, E6 | E. coli K-12 | ||||
| 622 | 8/1/2002 | CL610 | Charmain Courcelle | P1 transduction from TH457 | tus::kan | l-, rph-1, recD1011, argA81::Tn10, tus::kan | no | tn5 maybe?? | Box2, E7 | E. coli K-12 | |||
| 623 | 8/1/2002 | CL610 | Charmain Courcelle | P1 transduction from TH457 | tus::kan | l-, rph-1, recD1011, argA81::Tn10, tus::kan should be the same as CL622 but different isolate |
no | tn5 maybe?? | Box2, E8 | E. coli K-12 | |||
| 624 | 7/25/2002 | RW82 HL1061 | TK 603 | J Courcelle via Penny Beuning (MIT) | DumuDC595::cat, uvrA6 Mutat Res 281, 221-225 (1992); Mol Gen Genet 156, 121-131 (1977); can't tell what the rest of the genotype is from papers given. |
no | Box4, E9 | E. coli K-12 | |||||
| 625 | 7/25/2002 | GW8017 HL1061 | AB1157 | J Courcelle via Penny Beuning (MIT) | DumuDC595::cat | F- thr-1 ara-14 leuB6 Del(gpt-proA)62 lacY1 tsx-33 supE44 galK2 l- rac- hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 qsr-, DumuDC595::cat J. Bact. 1996 178:4400 |
no | Box4, F1 | E. coli K-12 | ||||
| 626 | 8/3/2002 | CL010 | Kin-Hoe Chow | P1 transduction from GW8017 | DumuDC595::cat | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recJ284::Tn10, rph?, DumuDC595::cat |
no | no | Box4, F2 | E. coli K-12 | |||
| 627 | 8/3/2002 | CL010 | Kin-Hoe Chow | P1 transduction from GW8017 | DumuDC595::cat | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recJ284::Tn10, rph?, DumuDC595::cat should be the same as CL626 but a different isolate |
no | no | Box4, F3 | E. coli K-12 | |||
| 628 | 8/3/2002 | CL581 | Kin-Hoe Chow | P1 transduction from CL007 | recF332::Tn3 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recQ6215 (sub cat883 for cdn19–606), recF332::Tn3 | no | Tn3 amp | Box4, F4 | E. coli K-12 | |||
| 629 | 8/3/2002 | CL581 | Kin-Hoe Chow | P1 transduction from CL007 | recF332::Tn3 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, recQ6215 (sub cat883 for cdn19–606), recF332::Tn3 should be the same as CL628 but a different isolate |
no | Tn3 amp | Box4, F4 | E. coli K-12 | |||
| 630 | 8/3/2002 | CL594 | Kin-Hoe Chow | P1 transduction from CL007 | recF332::Tn3 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recJ284::Tn10, rph?, recQ6215 (sub cat883 for cdn19–606), recF332::Tn3 |
no | Tn3 amp | Box4, F5 | E. coli K-12 | |||
| 631 | 8/3/2002 | CL594 | Kin-Hoe Chow | P1 transduction from CL007 | recF332::Tn3 | l-, thyA36, deoC2, IN(rrnD-rrnE)1, recJ284::Tn10, rph?, recQ6215 (sub cat883 for cdn19–606), recF332::Tn3 should be the same as CL630 but a different isolate |
no | Tn3 amp | Box4, F5 | E. coli K-12 | |||
| 632 | 8/10/2002 | CL001 | J Courcelle | P1 transduction from GW8017 | DumuDC595::cat | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, DumuDC595::cat |
no | no | Box4, F6 | E. coli K-12 | |||
| 633 | 8/10/2002 | CL001 | J Courcelle | P1 transduction from GW8017 | DumuDC595::cat | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, DumuDC595::cat should be the same as CL632 but a different isolate |
no | no | Box4, F7 | E. coli K-12 | |||
| 634 | 8/10/2002 | CL001 | J Courcelle | P1 transduction from CL605 | del(dinB)::Kan | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, del(dinB)::Kan |
no | no | Box4, F8 | E. coli K-12 | |||
| 635 | 8/10/2002 | CL001 | J Courcelle | P1 transduction from CL605 | del(dinB)::Kan | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, del(dinB)::Kan should be the same as CL632 but a different isolate ***not frozen yet |
no | no | Box4, F9 | E. coli K-12 | |||
| 636 | 8/10/2002 | CL001 | J Courcelle | P1 transduction from CL606 | del(polB)::omega spc | l-, thyA36, deoC2, IN(rrnD-rrnE)1, rph?, del(polB)::omega spc |
no | omega?? spc | Box4, G1 | E. coli K-12 |