Abramson

Office Phone:	(503) 725 - 3014
Office:	Room 330 Science Building 1
E-Mail:	<abramsonj@pdx.edu>

Research in this laboratory is aimed at understanding the molecular mechanism underlying contraction and relaxation in skeletal and cardiac muscle. Special attention is focused on the calcium release protein of the sarcoplasmic reticulum. The effects of sulfhydryl oxidizing and reducing agents has been used to unravel the mechanism by which this protein gates and controls the contractile state of muscle. The mechanism of oxidative damage induced by ischemia and old age is also presently being examined as is the mechanism underlying skeletal muscle fatigue. We have recently demonstrated that new more potent drugs designed to treat heart failure and muscle fatigue can be synthesized by modifying a drugs electron donor/acceptor properties. Designing new cardioprotective drugs has become a major focus of this laboratory. We have also recently identified a new protein involved in congenital skeletal muscle disorders. Our long term goal is to develop new methods for treating some forms of muscular dystrophy.

Jurynec, M.J., Xia, R., Mackrill, J.J., Gunther, D., Crawford, T., Flanigan, K.M., Abramson, J.J., Howard, M.T., Grunwald, D.J. Selenoprotein N is required for ryanodine receptor calcium release channel activity in human and zebrafish muscle. PNAS USA 105(34), 12485-90 (2008).

Nowak, D.B., Vatipalli, M., Abramson, J., Sánchez, E.J. Fabrication of a Versatile Substrates for Nanometer Relocation of Samples. Journal of Microscopy 230(1), 32-41 (2008).

Marinov, B.S., Olojo, R.O., Xia, R., Abramson, J.J. Non-thiol reagents regulate ryanodine receptor function by redox interactions that modify reactive thiols.Antioxid. Redox Signal. 9(5), 609-21 (2007).

Marinov, B.S., Bend, E.G., and Abramson, J.J. Reactions of Dye Free Radicals Reveal Weak Redox Properties of Drugs. Photochemistry and Photobiology 82(6), 1566-71 (2006).

Olojo, R.O., Xia, R.H., and Abramson, J.J. Spectrophotometric and fluorometric assay for superoxide ion using 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole Analytical Biochemistry 339, 338-344. (2005).

Cheong, E., Tumbev, V., Abramson, J., Salama, G., Stoyanovsky, D.A. Nitoxyl triggers release from sarcoplasmic reticulum by oxidizing ryanodine receptors. Cell Calcium 37, 87-96 (2005).

Xia, R., Ganther, H.E., Egge, A. and Abramson, JJ. Selenium Compounds modulate the calcium Release Channel/ Ryanodine receptor of rabbit skeletal muscle by oxidizing functional thiols. Biochemical Pharmacology 67/11, 2071-2079. (2004).

Xia, RH, Webb, J.A., Gnall, L.L.M., Cutler, K, and Abramson, J.J. Skeletal Muscle Sarcoplasmic Reticulum Contains a NADH Dependent Oxidase that generates Superoxide. Amer. J. Physiology Cell Physiology 285: C215-C221 (2003).

Favero, T,G, Webb, J., Papiez, M., Fisher, E., Trippichio, R.J., Broide, M., and Abramson, J.J. Hypochlorous acid activates and inhibits calcium release channel function from skeletal muscle sarcoplasmic reticulum. J. Applied Physiology. 94, 1387-1394 (2003).

Abramson, J.J., Mullen, S.P., Koehler, S., Mansoor, D., Anderson, P., Wamser, C.C., Swan, T.J., and Favero, T.G. o-Phthalaldehyde Activates the Ca2+ Release Mechanism from Skeletal Muscle Sarcoplasmic Reticulum. Arch. Biochem. & Biophys. 391, 235-244 (2001).

Abramson, J.J., Koehler, S., Mansoor, D., Anderson, P., Wamser, C.C., Swan, T.J., and Favero, T.G. o-Phthalaldehyde Activates the Calcium Release Mechanism from Skeletal Muscle Sarcoplasmic Reticulum. Arch. Biochem. & Biophys. 391, 235-244 (2001).

Xia, RH, Stangler, T., and Abramson, J.J. Skeletal Muscle Ryanodine Receptor is a Redox Sensor with a Well-defined Redox Potential, which is sensitive to Channel Modulators. J. Biol. Chem. 275, 36556-36561 (2000).

Salama, G., Menshikova, E.V., and Abramson, J.J. Molecular Interaction between Nitric Oxide and Ryanodine Receptors of Skeletal and Cardiac Sarcoplasmic Reticulum. Antioxidants and Redox Signaling 2 (1), 5-16 (2000).

Feng,W., Liu, G., Abramson, J. Xia, R., and Pessah, I.N. Site-selective Modification of Hyperreactive Thiols on Ryanodine Receptor Complex by Quinones. Mol. Pharmacol.. 55, 821-831 (1999).

Favero, T.G., Colter, D., Hooper, P. and Abramson, J.J.. Hypochlorous acid inhibits Ca²⁺ ATPase from skeletal muscle sarcoplasmic reticulum. Journal of Applied Physiology, 84(2), 425-430 (1998).

Zable, A.C., Favero, T.G., and Abramson, J.J. Glutathione modulates ryanodine receptor from skeletal muscle sarcoplasmic reticulum: Evidence for Redox control of the Ca²⁺ release mechanism. J. Biol. Chem. 272, 7069-7077 (1997).

Favero, T.G., Zable, A.C., Colter, D., and Abramson, J.J. Lactate Inhibits Ca²⁺Activated Ca²⁺ Channel Activity from Skeletal Muscle Sarcoplasmic Reticulum. J. Applied Physiol. 82, 447-452 (1997).

Favero, T.G., P. Hooper, D. Colter, and Abramson, J.J. Reactive oxygen species activate calcium release from skeletal muscle sarcoplasmic reticulum. Physiologist, 39, A15, 1996.

Hosenpud, J.D., Wright, J., Simpson, L., and Abramson, J.J. Caffeine Enhances Doxorubicin Cardiac Toxicity in an Animal Model. J. Cardiac Failure 1, 155-160. (1995).

Abramson, J.J., Zable, A.C., Favero, T.G., and Salama, G. Thimerosal Interacts with the Ca²⁺ release channel Ryanodine receptor from Skeletal Muscle Sarcoplasmic Reticulum. J. Biol. Chem. 270: 29644-29647. (1995).

Favero, T.G., Zable, A.C., and Abramson, J.J. Hydrogen Peroxide stimulates the Ca²⁺ release channel from skeletal muscle sarcoplasmic reticulum. J. Biol. Chem. 270: 25557-25563 (1995).

Favero, T., Zable, A.C., Bowman, M.B., Thompson, A., and Abramson, J.J. Metabolic end products inhibit sarcoplasmic reticulum Ca²⁺release and [3H]ryanodine binding. J. Applied Physiol. 78(5): 1665-1672 (1995).

Liu, G., Abramson, J.J., Zable, A.C., and Pessah, I.N. Direct evidence for the existence and functional role of Hyperreactive Sulfhydryls on the Ryanodine Receptor - Triadin complex selectively labeled by the coumarin maleimide 7-Diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin. Mol. Pharmac. 45: 189-200. (1994).

Favero, T.G. and Abramson, J.J. Thapsigargin-induced Ca²⁺ release from sarcoplasmic reticulum and asolectin vesicles. Cell Calcium 15 : 183-189 (1994)

Hadad, N., Zable, A.C., Abramson, J.J., and Shoshan-Barmatz, V. Ca²⁺ binding sites of the ryanodine receptor/ Ca²⁺ release channel of sarcoplasmic reticulum: low affinity binding site(s) as probed by Terbium fluorescence. J. Biol. Chem. 269: 24864-24869 (1994).

Buck, E., Zimanyi, I., Abramson, J.J., and Pessah, I.N. Ryanodine Stabilizes Multiple Conformational States of Skeletal Muscle Calcium Release. J. Biol. Chem. 267: 23560-23567 (1992)

Zimanyi, I., Buck, E., Abramson, J.J., Mack, M.M. and Pessah, I.N. Ryanodine Induces Persistent Inactivation of the Ca²⁺ Release Channel from Skeletal Muscle Sarcoplasmic Reticulum. Molecular Pharmac. 42: 1049-1057 (1992).

Salama, G., Abramson, J.J., and Pike, G.K. Sulphydryl reagents trigger Ca²⁺ release from the sarcoplasmic reticulum of skinned rabbit psoas fibres. J. Physiol. 454: 389-420. (1992).

Stuart, J., Pessah,I.N., Favero, T.G., Abramson, J.J. Photooxidation of skeletal muscle sarcoplasmic reticulum induces rapid calcium release. Arch. Biochem. Biophys. 292, 512-521 (1992).

Xiong, H., Buck, E., Stuart, J., Pessah, I.N.,Salama, G., Abramson, J.J. Rose bengal activates the Ca²⁺ release channel from skeletal muscle sarcoplasmic reticulum. Arch. Biochem. Biophys. 292, 522-528 (1992).

Salama, G., Abramson, J.J., Pike, G.K. Sulfhydryl reagents trigger Ca²⁺release from the sarcoplasmic reticulum of skinned rabbit psoas fibers. J. Physiol. 454, 389-420 (1992).